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Noelia Andollo, Marta Vicario, Rocio Herrero-Vanrell, Irene T. Molina-Martínez, Raquel Hernáez-Moya, Vanesa Freire, María-Celia Morales, Juan A Duran, Jaime Etxebarria; Effect Of A Bioadhesive As A Helper Of Blood Derivatives In The Re-epithelialization Of Rabbit Corneas. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3545.
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© ARVO (1962-2015); The Authors (2016-present)
To test in vivo the capability of a bioadhesive (hyaluronic acid) as a synergic drug combined with a blood derivative to improve the healing rate of corneal epithelial ulcers.
A 9-mm diameter of central corneal epithelium was mechanically scraped in 33 healthy eyes from New Zealand White rabbits, and the healing rate closure was analyzed. The following treatments were applied two times a day: 1) Phosphate Buffer Solution (PBS) as control, 2) 2,2% bioadhesive in PBS, 3) 90% Plasma Rich in Growth Factors (PRGF) in PBS, and 4) 90% PRGF together with 2,2% bioadhesive in PBS. To manufacture the blood derivative, eight millilitres of blood from rabbits was obtained. All rabbits were treated under the ARVO statements on the Care and Use of Animals in Vision Research.
No statistically significant differences in corneal wound healing rate among groups were found. 90% PRGF in PBS twice a day has no positive effect on the corneal wound healing rate compared to control. Conversely, 100% PRGF four times a day accelerates corneal epithelial healing (1977/D958 Poster, ARVO 2011). We could confirm the normal histology after the final epithelial healing in every group.
The bioadhesive by itself has not positive effect on the corneal wound healing rate. Moreover, the bioadhesive does not improve the effect of PRGF in healthy eyes, but it does not block the effect of the hemoderivative. This bioadhesive can not be used to lower the dosage of blood derivatives.
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