March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Mass Spectrometric Lipidomic Analyses of Alkali Exposed Corneal Tissue
Author Affiliations & Notes
  • Ashley M. Crane
    Department of Ophthalmology, Univ of Miami-Bascom Palmer Eye Inst, Miami, Florida
  • Andrew Coggin
    Department of Ophthalmology, Univ of Miami-Bascom Palmer Eye Inst, Miami, Florida
  • Hong-Uyen Hua
    Department of Ophthalmology, Univ of Miami-Bascom Palmer Eye Inst, Miami, Florida
  • Katyayini Aribindi
    Department of Ophthalmology, Univ of Miami-Bascom Palmer Eye Inst, Miami, Florida
  • Byron L. Lam
    Ophthalmology, Bascom Palmer Eye Institute, Miami, Florida
  • Sanjoy K. Bhattacharya
    Bascom Palmer Eye Institute, Univ of Miami Miller Sch of Med, Miami, Florida
  • Footnotes
    Commercial Relationships  Ashley M. Crane, None; Andrew Coggin, None; Hong-Uyen Hua, None; Katyayini Aribindi, None; Byron L. Lam, None; Sanjoy K. Bhattacharya, None
  • Footnotes
    Support  Supported by DOD grant W81XWH-09-1-0674 (Project 2.2), a career award and unrestricted funds from Research to Prevent Blindness and NIH core grant P30-EY14801.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3548. doi:
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      Ashley M. Crane, Andrew Coggin, Hong-Uyen Hua, Katyayini Aribindi, Byron L. Lam, Sanjoy K. Bhattacharya; Mass Spectrometric Lipidomic Analyses of Alkali Exposed Corneal Tissue. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3548.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To determine whether exposure to sodium hydroxide results in predictable changes in phospholipids in corneal tissue. To determine whether phospholipid profile changes correlate to exposure duration.

Methods: : Enucleated bovine (n= 40) and cadaver human eyes (n= 6) were procured from Just Meats, Chillicothe, Ohio and Florida Lion Eye Bank, Miami, Florida respectively and exposed to 0 (control), 0.2, 0.5 and 11M sodium hydroxide. The corneas were excised and lipids were extracted using the Bligh and Dyer method, and re-suspended in Isoproyl, Acetonitrile solution. Shotgun lipidomic was performed with a TSQ Quantum Access Max instrument. Briefly, positive mode precursor ion scan (PIS) for phosphocholines (PC; product m/z of 184) and negative mode PIS for phosphoinositol (PI; product m/z 241), phosphoethanolamine (PE; product m/z of 196) and neutral loss scan (NLS) for phosphoserine (PS; NL of 87.1) was used. The samples were infused and scanned for one minute between 200 m/z to 1000 m/z. Ratiometric quantification was performed using quantitative standards for each lipid class. This research adhered to ARVO statement for use of animals in research and Helsinki Declaration.

Results: : We found degradation of phospholipids of all classes: Phosphocholines, Phosphoethanolamines, Phosphoserines and Phosphoinositols. Furthermore, duration of exposure provided different amounts of lysophospholipids likely to be the product of phospholipids.

Conclusions: : Alkali exposure duration does indeed modify the corneal lipid profile.

Keywords: cornea: basic science • trauma • wound healing 
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