March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Long-Term In Vivo Biocompatibility of UV-Cross-Linked Hyaluronic Acid as Vitreous Replacement - 12 Months Results
Author Affiliations & Notes
  • Martin S. Spitzer
    Ophthalmology, Tuebingen University Eye Center, Tuebingen, Germany
  • Charlotte Schramm
    Ophthalmology, Univ Eye Hospital Tuebingen, Tuebingen, Germany
  • Tomasz Mlynczak
    Ophthalmology, Tuebingen University Eye Center, Tuebingen, Germany
  • Maximilian Schultheiss
    Departement of Ophthalmology, University of Tuebingen, Tuebingen, Germany
  • Sven Schnichels
    University Eye Hosp Tuebingen, Centre for Ophthalmology, Tuebingen, Germany
  • Peter Szurman
    Ophthalmology, Tuebingen University Eye Center, Tuebingen, Germany
  • Footnotes
    Commercial Relationships  Martin S. Spitzer, None; Charlotte Schramm, None; Tomasz Mlynczak, None; Maximilian Schultheiss, None; Sven Schnichels, None; Peter Szurman, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3767. doi:https://doi.org/
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      Martin S. Spitzer, Charlotte Schramm, Tomasz Mlynczak, Maximilian Schultheiss, Sven Schnichels, Peter Szurman; Long-Term In Vivo Biocompatibility of UV-Cross-Linked Hyaluronic Acid as Vitreous Replacement - 12 Months Results. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3767. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To test the long-term biocompatibility and the long-term stability of UV-cross-linked hyaluronic acid biopolymers as vitreous replacement in rabbit eyes.

Methods: : Hyaluronic acid was UV cross-linked using a modified protocol. The vitreous was removed by pars plana vitrectomy in 8 rabbit eyes and replaced by injection of the cross-linked biogels. ERG, slit lamp examination, funduscopy, OCT and measurement of intraocular pressure were performed at regular intervals postoperatively. The eyes were enucleated 6 to 12 months after surgery and histology was assessed for signs of cytotoxicity.

Results: : There were no signs of intraocular inflammation and intraocular pressure was normal during the entire postoperative period. Electrophysiology, histology, OCT and immunohistochemistry demonstrated an excellent tissue biocompatibility. The biogels remained in place for up to 6 months without losing optical clarity or elasticity. At 9 months the hydrogels started to dissolve. After 12 months the biogels had completely disappeared from the vitreous cavity.

Conclusions: : The results demonstrate the potential and the long-term biocompatibility of cross-linked hyaluronic acid biopolymers as a long-term vitreous replacement

Keywords: vitreous substitutes • vitreoretinal surgery • vitreous 
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