Purchase this article with an account.
Samin Hong, Yoko Iizuka, Chan Yun Kim, Gong Je Seong; Isolation Of Primary Mouse Retinal Ganglion Cells By Immunopanning-Magnetic Separation Method. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3837.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To verify the isolation method of primary mouse retinal ganglion cells (RGCs) by immunopanning-magnetic separation method.
Retinal cell suspension was obtained from one to four day-old ICR mouse eyeballs. Primary RGCs were isolated using three methods: 1) direct magnetic separation method, 2) two step immunopanning method, and 3) immunopanning-magnetic separation method.
As determined by Immunocytochemical staining and Western immunoblots, the primary RGCs isolated using direct magnetic separation method were contaminated by syntaxin-positive retinal amacrine cells; the cells isolated using two step immunopanning method were mixed by GFAP-positive retinal glial cells; the RGCs isolated using immunopanning-magnetic separation method showed the highest purity compared to other methods.
Immunopanning-magnetic separation method is a useful method for isolation of primary mouse RGCs in vitro.
This PDF is available to Subscribers Only