March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Oxidative Stress Markers in Patients with Different types of Glaucoma
Author Affiliations & Notes
  • Fabian S Lerner
    School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
    Fundacion para el Estudio del Glaucoma, Buenos Aires, Argentina
  • Sandra M. Ferreira
    School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Claudia Reides
    School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Susana Llesuy
    School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
  • Footnotes
    Commercial Relationships  Fabian S Lerner, None; Sandra M. Ferreira, None; Claudia Reides, None; Susana Llesuy, None
  • Footnotes
    Support  UBA 948
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3849. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Fabian S Lerner, Sandra M. Ferreira, Claudia Reides, Susana Llesuy; Oxidative Stress Markers in Patients with Different types of Glaucoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3849.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To determine oxidative stress markers in blood of patients with different types of glaucoma.

Methods: : Patients with primary open angle glaucoma (POAG, n=16), pseudoexfoliation glaucoma (PEXG, n=15) and controls (n= 16) were evaluated. The following markers were determined in red blood cells: chemiluminescence (CL), the activities of antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX); and the total antioxidant potential (TRAP) was determined in plasma.

Results: : SOD activity was significant decreased in PEXG patients (0.37 ± 0.05 U SOD/ mg protein) compared to controls (0.70 ± 0.20 USOD/ mg protein; p< 0.01). SOD activity was 0.19 ± 0.12 USOD/ mg protein in POAG patients (p<0.001). CAT levels were 1.3 ± 0.1 pmol/ mg protein in PEXG (p<0.001), 1.4 ± 0.1 pmol/ mg protein in POAG (p<0.01) (control levels 2.1 ± 0.2 pmol/ mg protein). CL in PEXG (353 ± 25 cpm/ mg haemoglobin), was higher than controls (139 ± 10 cpm/ mg haemoglobin; p<0.001). CL in POAG was 143 ± 17 cpm/ mg haemoglobin. TRAP levels of GPEX patients were 250 ± 17 µM Trolox (p<0.01), POAG patients 315 ± 19 µM Trolox (ns) (control values 338 ± 15 µM).

Conclusions: : A significantly reduced levels of non-enzymatic antioxidant (TRAP), a decreased in SOD and CAT activities and an increased of prooxidants (CL) were observed in pseudoexfoliation glaucoma. Meanwhile in POAG there were only a decrease in antioxidant enzymes activities. TRAP and CL could be used as biomarkers in both types of glaucoma.

Keywords: clinical laboratory testing • clinical (human) or epidemiologic studies: risk factor assessment • oxidation/oxidative or free radical damage 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×