March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Evaluation Of Oxidative DNA Damage And Its Repair In Pathogenesis Of Primary Open-angle Glaucoma
Author Affiliations & Notes
  • Ireneusz Majsterek
    Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland
  • Magdalena Cuchra
    Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland
  • Karolina Przybylowska
    Clinical Chemistry and Biochemistry, Medical University of Lodz, Lodz, Poland
  • Mira Gacek
    Ophtlamology and Eye Clinic, Medical University of Warsaw, Warsaw, Poland
  • Anna Kaminska
    Ophtlamology and Eye Clinic, Medical University of Warsaw, Warsaw, Poland
  • Jerzy Szaflik
    Ophtlamology and Eye Clinic, Medical University of Warsaw, Warsaw, Poland
  • Jacek P. Szaflik
    Ophtlamology and Eye Clinic, Medical University of Warsaw, Warsaw, Poland
  • Footnotes
    Commercial Relationships  Ireneusz Majsterek, None; Magdalena Cuchra, None; Karolina Przybylowska, None; Mira Gacek, None; Anna Kaminska, None; Jerzy Szaflik, None; Jacek P. Szaflik, None
  • Footnotes
    Support  This work was supported by grants N N402 375838 and N N402 248936 from Polish Ministry of Science and Higher Education.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3875. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ireneusz Majsterek, Magdalena Cuchra, Karolina Przybylowska, Mira Gacek, Anna Kaminska, Jerzy Szaflik, Jacek P. Szaflik; Evaluation Of Oxidative DNA Damage And Its Repair In Pathogenesis Of Primary Open-angle Glaucoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3875.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Glaucoma is a heterogeneous group of ocular disorder, which is characterized by progressive degeneration of optic nerve axon. Oxidative stress may play important role in glaucoma development, what suggests an important role of base excision repair (BER) pathway responsible for removing of oxidative DNA lesions. The aim of study was to evaluate the level of oxidative DNA lesions and its repair in peripheral lymphocytes of glaucoma patients.

Methods: : The study was performed on group of 10 patients with primary open-angle glaucoma (POAG) and 10 healthy subjects. An alkaline comet assay was used to measure DNA damage of oxidized purines as glicosylo-formamidoglicosylase (Fpg) sites, and oxidized pirmidines as endonuclease III (Nth) sites. We measured endogenous as well as exogenous DNA damage after 10 μM hydrogen peroxide treatment (H2O2). The efficiency of DNA repair was measured within 120 min of post treatment incubation with hydrogen peroxide.

Results: : Endogenous level of oxidative DNA damage in POAG patients was found to be statistically higher than the controls (P<0.001). Moreover, lymphocytes isolated from POAG patients with were more susceptible for oxidative DNA lesions induced by hydrogen peroxide than control subjects (P<0.001). Finally, we found an impaired repair of oxidative DNA lesions in POAG patients as compared to healthy controls (P<0.05).

Conclusions: : In conclusion our data revealed that oxidative stress might play an important role in POAG development and an impaired BER repair may be critical factor in glaucoma pathogenesis. Therefore, we suggested that the modulation of a pro-oxidant/antioxidant status might be a relevant target for prevention and therapy of glaucoma patients.This work was supported by grants N N402 375838 and N N402 248936 from Polish Ministry of Science and Higher Education.

Keywords: genetics • optic nerve • neuroprotection 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×