March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Toll-like Receptor Subtype Tlr5 Expression In Human Conjunctival Epithelium In Various Cicatricial Corneal Conjunctival Disorders
Author Affiliations & Notes
  • Keiko Yamada
    Kyoto Prefectural University of Medicine, Tokushima, Japan
  • Mayumi Ueta
    Ophthalmology, Kyoto Prefectural Univ of Med, Doshisha, Uji, Japan
  • Chie Sotozono
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Norihiko Yokoi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Tsutomu Inatomi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-Ku, Japan
  • Shigeru Kinoshita
    Ophthalmology, Kyoto Prefectural Univ of Med, Kamigyo-Ku, Japan
  • Footnotes
    Commercial Relationships  Keiko Yamada, None; Mayumi Ueta, None; Chie Sotozono, None; Norihiko Yokoi, None; Tsutomu Inatomi, None; Shigeru Kinoshita, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3985. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Keiko Yamada, Mayumi Ueta, Chie Sotozono, Norihiko Yokoi, Tsutomu Inatomi, Shigeru Kinoshita; Toll-like Receptor Subtype Tlr5 Expression In Human Conjunctival Epithelium In Various Cicatricial Corneal Conjunctival Disorders. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3985.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Toll-like receptors (TLRs), pattern-recognition receptors that sense conserved pathogen-associated molecular patterns (PAMPs), are the key receptors for the recognition of microbes. TLR5 recognizes bacterial flagellin, a component protein of bacterial flagella. Flagella are present in both gram-positive and gram-negative bacteria, and are essential for bacterial motility, invasion, and chemotaxis. We previously reported that human ocular-surface epithelial cells, both corneal and conjunctival, express TLR5 and secrete pro-inflammatory cytokines in response to flagellin derived from pathogenic ocular bacteria, but not from non-pathogenic ocular bacteria. The purpose of this present study was to examine the expression of TLR5 in various ocular surface disorders.

Methods: : Immunohistochemistry was performed on nearly normal conjunctival tissues obtained during surgery for conjunctivochalasis as a control, and on human conjunctival tissues obtained from patients undergoing surgical reconstruction of the ocular surface due to chemical eye burns, Stevens-Johnson syndrome (SJS), or ocular cicatricial pemphigoid (OCP). For TLR5 staining, mouse anti-human TLR5 monoclonal antibody (mAb; Abcam, Cambridge, UK) was used.

Results: : TLR5 protein was consistently and abundantly expressed in human conjunctival epithelium and detected only at the basal- and wing sites in conjunctival epithelium obtained from the patients with conjunctivochalasis, indicating its spatially selective presence on the basolateral- but not the apical side. On the other hand, in the conjunctival epithelium obtained from the patients with chemical eye burns, SJS, and OCP, the TLR5 protein was detected at not only the basolateral side but also in the near apical side.

Conclusions: : The findings of this study suggest that TLR5 protein might be up-regulated in the conjunctival epithelium of patients with chemical eye burns, SJS, or OCP.

Keywords: cornea: epithelium • conjunctiva 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×