Filaggrin Mutations Do Not Associate With Chronic Allergic Keratoconjunctivitis

Abstract

 

Purpose:

 

Filaggrin gene (FLG) mutations are predisposing genetic factor for atopic dermatitis (AD). We investigated the association between FLG mutation and atopic keratoconjunctivitis (AKC) or vernal keratoconjunctivitis (VKC) using Japanese population.

 

Methods:

 

4 common FLG mutations in Japanese (S2554X, S2889X, S3296X, 3321delA) were genotyped using DNA samples obtained from 69 cases ( 44 AKC and 25 VKC) and 93 healthy controls. Direct sequence was carried out by dye-terminator methods. cDNA was made from conjunctival epithelial cells and FLG mRNA expression was examined by PCR.

 

Results:

 

The results of genotyping were summarized in the following table. P:P-value of Χ²-test for dominant models (AA vs Aa+aa). No significant association was observed between FLG mutation and chronic allergic keratoconjunctivitis. RT-PCR revealed FLG mRNA expression in conjunctival epithelial cells.

 

Conclusions:

 

Our result showed no association between FLG mutations and chronic allergic keratoconjunctivitis in Japanese population.  

 

Keywords: conjunctivitis • genetics