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Satoshi Iwamoto, Akira Matsuda, Nobuyuki Ebihara, Kanji Hori, Toshinari Funaki, Norihiko Yokoi, Satoshi Kawasaki, Akira Murakami; Filaggrin Mutations Do Not Associate With Chronic Allergic Keratoconjunctivitis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3997.
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© ARVO (1962-2015); The Authors (2016-present)
Filaggrin gene (FLG) mutations are predisposing genetic factor for atopic dermatitis (AD). We investigated the association between FLG mutation and atopic keratoconjunctivitis (AKC) or vernal keratoconjunctivitis (VKC) using Japanese population.
4 common FLG mutations in Japanese (S2554X, S2889X, S3296X, 3321delA) were genotyped using DNA samples obtained from 69 cases ( 44 AKC and 25 VKC) and 93 healthy controls. Direct sequence was carried out by dye-terminator methods. cDNA was made from conjunctival epithelial cells and FLG mRNA expression was examined by PCR.
The results of genotyping were summarized in the following table. P:P-value of Χ2-test for dominant models (AA vs Aa+aa). No significant association was observed between FLG mutation and chronic allergic keratoconjunctivitis. RT-PCR revealed FLG mRNA expression in conjunctival epithelial cells.
Our result showed no association between FLG mutations and chronic allergic keratoconjunctivitis in Japanese population.
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