April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Regulation Of The Akt Signaling Transduction Pathway By αA-crystallin
Author Affiliations & Notes
  • Lili Gong
    Biochemistry & Molecular Biology,
    University of Nebraska Medical Center, Omaha, Nebraska
  • Jin-Ping Liu
    Biochemistry & Molecular Biology,
    University of Nebraska Medical Center, Omaha, Nebraska
  • Mi Deng
    Biochemistry & Molecular Biology,
    University of Nebraska Medical Center, Omaha, Nebraska
  • Weike Ji
    Biochemistry & Molecular Biology,
    University of Nebraska Medical Center, Omaha, Nebraska
  • Haili Ma
    Biochemistry & Molecular Biology,
    University of Nebraska Medical Center, Omaha, Nebraska
  • David W. Li
    Biochemistry & Molecular Biology,
    Ophthalmology & Visual Sciences,
    University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships  Lili Gong, None; Jin-Ping Liu, None; Mi Deng, None; Weike Ji, None; Haili Ma, None; David W. Li, None
  • Footnotes
    Support  EY018380
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3930. doi:https://doi.org/
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    • Get Citation

      Lili Gong, Jin-Ping Liu, Mi Deng, Weike Ji, Haili Ma, David W. Li; Regulation Of The Akt Signaling Transduction Pathway By αA-crystallin. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3930. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Cataract is a major ocular disease that causes blindness in many developing countries. Cell apoptosis plays a critical role in cataractogenesis. αA-crystallin (αA) is a structural protein in lens, and it also belongs to the small heat shock protein (Hsp) family which exerts anti-apoptotic function. Mice deficient in αA (αA-/-) develop cataracts in which cell apoptosis was significant detected. AKT is an important survival signal molecule in many cell types. The purpose of the presented study is to explore the possible anti-apoptotic mechanism of αA through regulating the AKT signaling pathway.

Methods: : Surface plasma resonance analysis and co-immunoprecipitation assay were used to investigate the interactions between αA and AKT in vitro. Electronic gel mobility shifting assay, reverse transcription polymerase chain reaction and Western-blot analysis were utilized to study the activation of AKT signaling pathway in the cellular level. Both αA-overexpressing lens epithelial cell (LEC) lines and LECs derived from αA-/- mice were used as cell models to study the AKT signaling pathway. Cell viability, gene expression and kinase activity were compared between αA-overexpressing and vector transfected LECs during H2O2 treatment.

Results: : Our result showed that αA specifically bound to AKT in vitro. Significantly reduced activity of AKT and its downstream targets GSK3β and CREB were detected in LECs derived from αA-/- mice. Overexpression of αA in LECs increased cell viability during H2O2 treatment, which depended on constitutive activation of AKT, GSK3β and CREB.

Conclusions: : These observations suggest that αA promotes LECs survival by directly activating the AKT signaling pathway.

Keywords: crystallins • phosphorylation • apoptosis/cell death 
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