April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Role of Vascular Endothelial Growth Factor Induced by Photodynamic Therapy
Misa Suzuki; Yoko Ozawa; Shunsuke Kubota; Manabu Hirasawa; Seiji Miyake; Toshio Narimatsu; Kazuo Tsubota; Kazuaki Kadonosono; Kousuke Noda; Susumu Ishida
Author Affiliations & Notes
  • Misa Suzuki
    Ophthalmology and Visual Science, Yokohama City University, Yokohama, Japan
    Ophthalmology, Keio University, Tokyo, Japan
  • Yoko Ozawa
    Ophthalmology, Keio University, Tokyo, Japan
  • Shunsuke Kubota
    Department of Ophthalmology, Keio University, Shinjyuku-ku, Japan
  • Manabu Hirasawa
    Ophthalmology, Keio University, Shinjuku-ku, Japan
  • Seiji Miyake
    Ophthalmology, Keio University, Tokyo, Japan
  • Toshio Narimatsu
    Ophthalmology, Keio University, Tokyo, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio University, Tokyo, Japan
  • Kazuaki Kadonosono
    Ophthalmology and Visual Science, Yokohama City University Medical Center, Yokohama, Japan
  • Kousuke Noda
    Ophthalmology, Hokkaido Univ Grad Sch of Med, Sapporo, Japan
  • Susumu Ishida
    Ophthalmology, Hokkaido Univ Grad Sch of Med, Sapporo, Japan
  • Footnotes
    Commercial Relationships  Misa Suzuki, Novartis (R); Yoko Ozawa, Novartis (C, R); Shunsuke Kubota, None; Manabu Hirasawa, None; Seiji Miyake, None; Toshio Narimatsu, None; Kazuo Tsubota, None; Kazuaki Kadonosono, None; Kousuke Noda, None; Susumu Ishida, None
  • Footnotes
    Support  Novartis
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3935. doi:
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      Misa Suzuki, Yoko Ozawa, Shunsuke Kubota, Manabu Hirasawa, Seiji Miyake, Toshio Narimatsu, Kazuo Tsubota, Kazuaki Kadonosono, Kousuke Noda, Susumu Ishida; Role of Vascular Endothelial Growth Factor Induced by Photodynamic Therapy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3935.

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Abstract

Purpose: : To investigate the role of vascular endothelial growth factor (VEGF) induced by photodynamic therapy (PDT) in the retina.

Methods: : Six week-old C57/B6 male mice were prepared. Verteporfin was injected from their tail veins and the intact retinas were exposed to the laser to activate the drug15 minutes after the injection. The level of VEGF in the retina after PDT was measured using ELISA at each time point and compared with mice with no treatment. Next, VEGF inhibitor, VEGFRc/fusion protein, or control vehicle was injected intravitreally immediately after PDT or 7 days after PDT. Histological changes were evaluated both by hematoxylin eosin (HE) staining and TUNEL staining.

Results: : The level of VEGF was increased in the retina peaking at 3 hours after PDT, and returned to the normal level by 3 days. In the retina treated with VEGF inhibitor immediately after PDT, thickness of photoreceptor cell layer was reduced and the retinal degeneration was induced, compared with that treated with control vehicle after PDT. TUNEL staining 3 days after the treatment showed increased apoptotic cells in the retina of combined treatment with PDT and simultaneous VEGF inhibition. These histological changes were not observed when VEGFRc/fusion protein was injected in the eyes without PDT or 7 days after PDT.

Conclusions: : VEGF, transiently induced by PDT in the mouse retina, played a physiological role on retinal neuroprotection.

Keywords: photodynamic therapy • neuroprotection • cell survival 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2023 Association for Research in Vision and Ophthalmology.
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