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Misa Suzuki, Yoko Ozawa, Shunsuke Kubota, Manabu Hirasawa, Seiji Miyake, Toshio Narimatsu, Kazuo Tsubota, Kazuaki Kadonosono, Kousuke Noda, Susumu Ishida; Role of Vascular Endothelial Growth Factor Induced by Photodynamic Therapy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3935.
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To investigate the role of vascular endothelial growth factor (VEGF) induced by photodynamic therapy (PDT) in the retina.
Six week-old C57/B6 male mice were prepared. Verteporfin was injected from their tail veins and the intact retinas were exposed to the laser to activate the drug15 minutes after the injection. The level of VEGF in the retina after PDT was measured using ELISA at each time point and compared with mice with no treatment. Next, VEGF inhibitor, VEGFRc/fusion protein, or control vehicle was injected intravitreally immediately after PDT or 7 days after PDT. Histological changes were evaluated both by hematoxylin eosin (HE) staining and TUNEL staining.
The level of VEGF was increased in the retina peaking at 3 hours after PDT, and returned to the normal level by 3 days. In the retina treated with VEGF inhibitor immediately after PDT, thickness of photoreceptor cell layer was reduced and the retinal degeneration was induced, compared with that treated with control vehicle after PDT. TUNEL staining 3 days after the treatment showed increased apoptotic cells in the retina of combined treatment with PDT and simultaneous VEGF inhibition. These histological changes were not observed when VEGFRc/fusion protein was injected in the eyes without PDT or 7 days after PDT.
VEGF, transiently induced by PDT in the mouse retina, played a physiological role on retinal neuroprotection.
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