April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
In Vivo Retinal Layer Imaging in Rat Model of Retinopathy of Prematurity (ROP) using Adaptive Optics Spectral Domain Optical Coherence Tomography (AO-SDOCT)
Author Affiliations & Notes
  • Toco Y. Chui
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • James D. Akula
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • Daniel X. Hammer
    Physical Sciences Inc, Andover, Massachusetts
  • R D. Ferguson
    Physical Sciences Inc, Andover, Massachusetts
  • Ronald M. Hansen
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • Anne B. Fulton
    Ophthalmology, Children's Hospital Boston, Boston, Massachusetts
  • Footnotes
    Commercial Relationships  Toco Y. Chui, None; James D. Akula, None; Daniel X. Hammer, Physical Sciences Inc (E), simultaneous SLO/OCT imaging in the multimodal format, Physical Sciences Inc (P); R. D. Ferguson, Physical Sciences Inc (E), Tracking and LSO imaging system, Physical Sciences Inc (P); Ronald M. Hansen, None; Anne B. Fulton, None
  • Footnotes
    Support  NIH Grants EY109575 (Fulton), RC1 EY020308 (Akula), and EY018986 (Hammer); Massachusetts Lions Eye Research Fund (Hansen)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3992. doi:
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      Toco Y. Chui, James D. Akula, Daniel X. Hammer, R D. Ferguson, Ronald M. Hansen, Anne B. Fulton; In Vivo Retinal Layer Imaging in Rat Model of Retinopathy of Prematurity (ROP) using Adaptive Optics Spectral Domain Optical Coherence Tomography (AO-SDOCT). Invest. Ophthalmol. Vis. Sci. 2011;52(14):3992.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To evaluate the retinal layers in the Penn et al. (1994) rat model of ROP using high resolution AO-SDOCT and to make images of the ONH.

 
Methods:
 

A multimodal adaptive optics retinal imaging system (Hammer et al., IOVS 2010; E-Abstract 3455) was used to image the retinal layers of ROP (n=5) and room-air-reared (RAR) control rats (n=7) (age 76±7day). Vertical SDOCT line scans were obtained at 2° from the ONH in both eyes (Fig. 1). On the scans, the ILM, OPL, and RPE were segmented using custom software. 6°x6° raster scans, centered at the optic disc, were obtained in 6 additional RAR rats. 3D representations of the optic disc were then created from the raster scans as shown in Fig. 2.

 
Results:
 

In respective ROP and RAR rats, the mean±SD (µm) total retinal thickness (ILM to RPE) was 197.70±8.27 and 224.66±16.50; photoreceptor layer thickness (OPL to RPE) was 98.75±10.38 and 107.21±15.92; postreceptor layer thickness (ILM to INL) was 98.95±6.25 and 117.45±9.07. The total retinal and postreceptor thicknesses were significantly thinner in the ROP than in the RAR rats (P<0.05); the photoreceptor layer thickness did not differ significantly between groups (P=0.157). Sclera and choroid were clearly visualized in some of the SDOCT scans. The ONH, its peripapillary vasculature and remnants of hyaloid vasculature were readily visualized.

 
Conclusions:
 

The OCT recapitulated, in vivo, histology showing attenuation in the postreceptor retina in ROP rats (Akula et al., Doc Ophthalmol 2010). This noninvasive approach, however, is applicable to longitudinal studies of neural and vascular elements.  

 
Keywords: retinopathy of prematurity • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • retina 
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