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Yasuyuki Yamauchi, Keisuke Kimura, Tsuyoshi Agawa, Rintaro Tsukahara, Maki Mishima, Naoyuki Yamakawa, Hiroshi Goto; Correlation Between High-resolution Optical Coherence Tomography Images And Histopathology In An N-methyl-n-nitrosourea Induced Retinal Degeneration Rat Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4041.
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© ARVO (1962-2015); The Authors (2016-present)
Recent research on macular disease has prompted investigation into the condition of the intersection between the inner and outer segments (IS/OS) and its relationship with retinal photoreceptor abnormalities. Because the correlation between optical coherence tomography (OCT) images and histopathology is unclear, we compared these in an N-methyl-N-nitrosourea (MNU)-induced photoreceptor degeneration rat model.
(MNU) (60 mg/kg), which is toxic to photoreceptors, was injected in 12 Brown Norway rat. After MNU administration, each 3 rats were used for histopathological study at 3hr, 6hr, 24hr and 1 week. Two healthy rats served as controls. OCT images of the fundus were taken before euthanization.
Three hours after injections of MNU, the IS and OS was oedematous but still the borderline of IS/OS was recognized histopathologically and the IS/OS was depicted on the OCT images. Six hours after injections, the OS were still preserved, but the structure of the IS was destroyed or partially disorganized histopathologically, and the IS/OS was not observed on the OCT images. Twenty four hours after injections, IS/OS was totally disorganized histopathologically and IS/OS was not depicted at all on the OCT images. One week after injections, outer part of the retina was disappeared and IS/OS was not depicted at all on the OCT images.
The IS/OS on the OCT images probably reflected the histopathological alterations of the inner segment of the photoreceptor in an experimental photoreceptor degeneration model in rats.
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