April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Correlation Between High-resolution Optical Coherence Tomography Images And Histopathology In An N-methyl-n-nitrosourea Induced Retinal Degeneration Rat Model
Author Affiliations & Notes
  • Yasuyuki Yamauchi
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
    Ophthalmology, Kohsei Chuo Genral Hospital, Meguro-ku, Tokyo, Japan
  • Keisuke Kimura
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Tsuyoshi Agawa
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Rintaro Tsukahara
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Maki Mishima
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Naoyuki Yamakawa
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Hiroshi Goto
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships  Yasuyuki Yamauchi, None; Keisuke Kimura, None; Tsuyoshi Agawa, None; Rintaro Tsukahara, None; Maki Mishima, None; Naoyuki Yamakawa, None; Hiroshi Goto, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4041. doi:
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      Yasuyuki Yamauchi, Keisuke Kimura, Tsuyoshi Agawa, Rintaro Tsukahara, Maki Mishima, Naoyuki Yamakawa, Hiroshi Goto; Correlation Between High-resolution Optical Coherence Tomography Images And Histopathology In An N-methyl-n-nitrosourea Induced Retinal Degeneration Rat Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4041.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Recent research on macular disease has prompted investigation into the condition of the intersection between the inner and outer segments (IS/OS) and its relationship with retinal photoreceptor abnormalities. Because the correlation between optical coherence tomography (OCT) images and histopathology is unclear, we compared these in an N-methyl-N-nitrosourea (MNU)-induced photoreceptor degeneration rat model.

Methods: : (MNU) (60 mg/kg), which is toxic to photoreceptors, was injected in 12 Brown Norway rat. After MNU administration, each 3 rats were used for histopathological study at 3hr, 6hr, 24hr and 1 week. Two healthy rats served as controls. OCT images of the fundus were taken before euthanization.

Results: : Three hours after injections of MNU, the IS and OS was oedematous but still the borderline of IS/OS was recognized histopathologically and the IS/OS was depicted on the OCT images. Six hours after injections, the OS were still preserved, but the structure of the IS was destroyed or partially disorganized histopathologically, and the IS/OS was not observed on the OCT images. Twenty four hours after injections, IS/OS was totally disorganized histopathologically and IS/OS was not depicted at all on the OCT images. One week after injections, outer part of the retina was disappeared and IS/OS was not depicted at all on the OCT images.

Conclusions: : The IS/OS on the OCT images probably reflected the histopathological alterations of the inner segment of the photoreceptor in an experimental photoreceptor degeneration model in rats.

Keywords: imaging/image analysis: non-clinical • photoreceptors • pathology: experimental 
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