April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Expression Of VEGFR3, VEGF-C And Other Lymphangiogenic Markers During The Development Of The Human Choroid
Author Affiliations & Notes
  • Ping Hu
    Department of Anatomy, University of Sydney, Camperdown, Australia
  • Louise Baxter
    Department of Anatomy, University of Sydney, Camperdown, Australia
  • Frank Arfuso
    Department of Anatomy, University of Sydney, Camperdown, Australia
  • Sam Adamson
    Department of Anatomy, University of Sydney, Camperdown, Australia
  • Jan McColm
    Department of Anatomy, University of Sydney, Camperdown, Australia
  • Michele Madigan
    Department of Anatomy, University of New South Wales, Camperdown, Australia
  • Jane Dahlstrom
    ACT Pathology, Canberra, Australia
  • Mark Koina
    ACT Pathology, Canberra, Australia
  • Kari Alitalo
    University of Helsinki, Helsinki, Finland
  • Tailoi Chan-Ling
    Department of Anatomy, University of Sydney, Camperdown, Australia
  • Footnotes
    Commercial Relationships  Ping Hu, None; Louise Baxter, None; Frank Arfuso, None; Sam Adamson, None; Jan McColm, None; Michele Madigan, None; Jane Dahlstrom, None; Mark Koina, None; Kari Alitalo, None; Tailoi Chan-Ling, None
  • Footnotes
    Support  Baxter Charitable Foundation, Rebecca L. Cooper Medical Research Foundation, National Health & Medical Research Council of Australia
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4078. doi:
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      Ping Hu, Louise Baxter, Frank Arfuso, Sam Adamson, Jan McColm, Michele Madigan, Jane Dahlstrom, Mark Koina, Kari Alitalo, Tailoi Chan-Ling; Expression Of VEGFR3, VEGF-C And Other Lymphangiogenic Markers During The Development Of The Human Choroid. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4078.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To undertake a characterisztion of human lymphatic vascular formation, utilizing lymphatic specific markers, VEGFR3 and VEGFC during the development of the human choroid.

Methods: : Transverse and whole mount choroids were prepared from developmental human foetal eyes aged 12 to 20wg and young adult human aged 30 to 40 years. Triple label immunohistochemistry for Podoplanin, PROX-1, D2-40 and LYVE-1 for lymphatic endothelial cells (LECs), CD34 and CD39 for vascular endothelial cells (VECs) were utilized. VEGFC & R3, the VEGFs responsible for the lymphatic lineage, were also localised in the choroid. The receptor R3 as well as its ligand were also quantified using Western blots.

Results: : The topography of the developing lymphatic vessel structures showed that the lymph structures emanate from the optic nerve head of the human choroid.Structural evidence of lymphatic vessel structures as opposed to blood vessels: lymph sacs, high lymphatic endothelial cells (LECs) with lower density and much wider gaps along the vessel lumen than vascular endothelial cells (VECs) were found in 30 and 40yr old human choroids; very broad lumens with irregular calibre, 56-80um wide, and irregular branching patterns; double layered vessel walls; the lymph vessels have less cellular content in the lumen, some are nearly empty, which is consistent with their function of collecting fluid. Unique evidence for lymphatic vessel structure is a lymphatic valve, which was found in young adult choroid. Morphology suggestive of of lymphatic remodelling was shown by blending/merging between vessels, and lymphangiogenesis found in the 40yr old human choroid. Lymphatic marker+, LYVE-1A+ /VEGFR3+, microglia / macrophages were found in entire thickness of the 15wg choroid. Western Blots were performed for the Lymphatic markers Podoplanin / PROX-1A / LYVE-1A.

Conclusions: : Thus there appears to be an interaction between the lymphatic and vascular lineage in the developing human choroid and in the young adult human choroid.This newly identified lineage may play a role in choroidal neovascular diseases, and further understanding is needed

Keywords: choroid • choroid: neovascularization • age-related macular degeneration 
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