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Tadashi Ito, Yumiko Yokoi, Yukihiko Suzuki, Hiroya Ota, Miki Takada, Mitsuru Nakazawa; Mesurements Of Anti-oxidant Potentials In Vitreous Fluids Obtained From Patients With Various Vitreoretinal Diseases. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4083.
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Various eye diseases have relevance to oxidative stress. Especially, the retina constantly exposed to light is subject to oxidative stress. Because vitreous body contains ascorbic acid and glutathione, it may have an anti-oxidant potential. To reveal the role of vitreous body against oxidative stress, we measured anti-oxidant potentials in the vitreous fluids obtained from patients with various vitreoretinal diseases at the time of pars plana vitrectomy. In the abnormal status of retina including diabetic retinopathy (DR) , which is likely related to oxidative stress, vitreous body may have a protective role against progression of diseases.
Vitreous fluids were collected from patients who underwent vitrectomy at the Hirosaki University Hospital after obtaining informed consents. This study was approved by the ethics commitee of the Hirosaki University Hospital. The subjects were 18 eyes with macular hole (MH), 12 eyes with epiretinal membrane (ERM), 48 eyes with proliferative diabetic retinopathy (PDR), 9 eyes with central retinal vein occlusion (CRVO), and 26 eyes with retinal detachment (RD). Each vitreous fluid was immediately stored at -80°C until measurement. Anti-oxidant potential of vitreous fluid was defined as the amount of Fe3+ (µM) which was reduced to Fe2+ by 10µl vitreous fluid under thiocyanate using a free radical elective evaluator (FREETM).
Mean anti-oxidant potential (µM) were MH: 2516.3±532.0,ERM: 2315.1±362.2,PDR: 1788.5±362.2,CRVO: 2014.5±435.0 and RD: 2071±623.5. Significant differences were found between MH and PDR (P<0.001), CRVO (P=0.038), RD (P=0.024), respectively. No significant difference was obtained between MH and ERM.
Anti-oxidant potential was diminished in PDR, CRVO and RD, particularly in PDR. Overloaded oxdative stress may be partially canceled by vitreous body in these diseases.
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