April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Pe-11, A Peptide Derived From Chromogranin B, In The Rat Eye
Author Affiliations & Notes
  • Katrin Lorenz
    Department of Ophthalmology, Johannes Gutenberg-University Mainz, Mainz, Germany
  • Josef Troger
    Ophthalmology & Optometry, University of Innsbruck, Innsbruck, Austria
  • Oliver W. Gramlich
    Experimental Ophthalmology, University Medical Center, Mainz, Mainz, Germany
  • Franz H. Grus
    Experimental Ophthalmology, University Medical Center, Mainz, Germany
  • Reiner Fischer-Colbrie
    Department of Pharmacology,
    Medical University of Innsbruck, Innsbruck, Austria
  • Stephanie C. Joachim
    Experimental Ophthalmology, University of Mainz, Mainz, Germany
  • Eduard Schmid
    Ophthalmology & Optometry, Innsbruck Medical University, Innsbruck, Austria
  • Gertrud Haas
    Department of Ophthalmology,
    Medical University of Innsbruck, Innsbruck, Austria
  • Nikolaos Bechrakis
    Department of Ophthalmology,
    Medical University of Innsbruck, Innsbruck, Austria
  • Footnotes
    Commercial Relationships  Katrin Lorenz, None; Josef Troger, None; Oliver W. Gramlich, None; Franz H. Grus, None; Reiner Fischer-Colbrie, None; Stephanie C. Joachim, None; Eduard Schmid, None; Gertrud Haas, None; Nikolaos Bechrakis, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4102. doi:
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      Katrin Lorenz, Josef Troger, Oliver W. Gramlich, Franz H. Grus, Reiner Fischer-Colbrie, Stephanie C. Joachim, Eduard Schmid, Gertrud Haas, Nikolaos Bechrakis; Pe-11, A Peptide Derived From Chromogranin B, In The Rat Eye. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4102.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the presence and distribution of PE-11, a peptide derived from chromogranin B, in the rat eye.

Methods: : Newborn rats were subcutaneously injected with a single dosage of 50mg/kg capsaicin under the neck fold and after three months, particular eye tissues were dissected and the concentration of PE-11 was determined by radioimmunoassay. Then the distribution pattern of PE-11 was investigated in the rat eye and rat trigeminal ganglion by immunofluorescence.

Results: : Capsaicin pretreatment led to a 88.05 % (± 7.07) and a 64.26 % (± 14.17) decrease of the levels of PE-11 in the cornea and choroid/sclera, respectively, and to a complete loss in the iris/ciliary body complex compared to controls. Sparse nerve fibers were visualized in the corneal and uveal stroma, surrounding blood vessels at the limbus, ciliary body and choroid and in association with the dilator and sphincter muscle. Furthermore, immunoreactivity was present in the corneal endothelium. Glia was labeled in the retina and optic nerve. In the rat trigeminal ganglion, PE-11-like immunoreactivity was visualized in small sized ganglion cells with a diameter of up to 30µm.

Conclusions: : These data provide evidence that PE-11 is a constituent of capsaicin-sensitive sensory neurons innervating the rat eye and the distribution pattern is typically peptidergic in the peripheral innervation but in the retina completely atypical for neuropeptides and unique.

Keywords: immunohistochemistry • neuropeptides • retina: neurochemistry 

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