April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Localization of Potassium Channels Kir2.1 and Kir4.1 in Monkey Retina
Author Affiliations & Notes
  • Louvenia D. Carter-Dawson
    Ophthalmology & Visual Science, The Univ of Texas Medical School at Houston, Houston, Texas
  • Yenabi J. Keflemariam
    Ophthalmology & Visual Science, The Univ of Texas Medical School at Houston, Houston, Texas
  • Laura J. Frishman
    College of Optometry,
    University of Houston, Houston, Texas
  • Ronald S. Harwerth
    Optometry,
    University of Houston, Houston, Texas
  • Footnotes
    Commercial Relationships  Louvenia D. Carter-Dawson, None; Yenabi J. Keflemariam, None; Laura J. Frishman, None; Ronald S. Harwerth, None
  • Footnotes
    Support  NIH/NEI grants EY01139, EY10608 and P30 07551 and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4132. doi:https://doi.org/
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      Louvenia D. Carter-Dawson, Yenabi J. Keflemariam, Laura J. Frishman, Ronald S. Harwerth; Localization of Potassium Channels Kir2.1 and Kir4.1 in Monkey Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4132. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate distribution of Kir2.1 and Kir4.1 inwardly rectifying potassium channels in primate retina.

Methods: : Posterior segments from monkey eyes were fixed in 4% paraformaldehyde in phosphate buffer. Retina samples were taken along the horizontal meridian of each eye. The sections were exposed to polyclonal antibody to Kir2.1 or Kir4.1 and monoclonal antibody to glutamine synthetase (GS), a marker for Müller cells. Antibody binding in retina was visualized with a Cy3 conjugated secondary antibody. Images of immunolabeled retina were captured by confocal microscopy and converted to 3D-projections using Image J software.

Results: : Kir4.1 was broadly expressed in Müller cells, in the somas in the inner nuclear layer (INL), processes and trunks in the inner plexiform layer, end-feet, and surrounding blood vessels. Kir2.1was predominately localized to bipolar cell dendrites, somas, axons and terminals. In several samples, Kir2.1 was detected in some neurons in the ganglion cell layer and neurons other than bipolar cells in the INL. GS co-localized with Kir4.1, but not with Kir2.1.

Conclusions: : The distribution of Kir4.1 channels in monkey retina is consistent with reports for mouse and rat, thus indicating similar distributions across mammalian species. Kir2.1 distribution in monkey retina is similar to that reported for rat, except for prominent labeling of bipolar cell dendrites in monkey. Müller cells are reported to express Kir2.1 in mouse, but the lack of co-localization between GS and Kir2.1 indicates that these cells may not express Kir2.1 in monkey. Kir2.1 and Kir4.1 contribute to the generation of scotopic and photopic negative responses in the rat ERG. Since their distribution is similar in monkey, investigating alterations in their expression in glaucoma monkey retinas may provide insight into the changes in these negative ERG responses observed in monkeys with experimental glaucoma.

Keywords: ion channels • retina: proximal (bipolar, amacrine, and ganglion cells) • immunohistochemistry 
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