April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
A New Mucoadhesive Ophthalmic Hydrocortisone Formulation. "In Vivo" Measurement of Precorneal Residence Time in Rabbits
Author Affiliations & Notes
  • Sebastiano Mangiafico
    Medivis Italy, Catania, Italy
  • Danilo Aleo
    Medivis Italy, Catania, Italy
  • Maria G. Saita
    Medivis Italy, Catania, Italy
  • Martina Battaglia
    Centro Diagnostico Oculistico, Siracusa, Italy
  • Sergio Mangiafico
    Medivis Italy, Catania, Italy
  • Melina G. Cro
    Medivis Italy, Catania, Italy
  • Footnotes
    Commercial Relationships  Sebastiano Mangiafico, Medivis, Italy (E); Danilo Aleo, Medivis, Italy (E); Maria G. Saita, Medivis, Italy (E); Martina Battaglia, None; Sergio Mangiafico, Medivis, Italy (E); Melina G. Cro, Medivis, Italy (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4319. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Sebastiano Mangiafico, Danilo Aleo, Maria G. Saita, Martina Battaglia, Sergio Mangiafico, Melina G. Cro; A New Mucoadhesive Ophthalmic Hydrocortisone Formulation. "In Vivo" Measurement of Precorneal Residence Time in Rabbits. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4319.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : Objective of the study was developing a new Hydrocortisone/Hyaluronic Acid (MDV/HA) low-viscosity topical ophthalmic formulation highly mucoadhesive on the ocular surface.

Methods: : Two formulations based on 0.335% Hydrocortisone (HY) sodium phosphate were prepared: MDV (containing the corticosteroid in phosphate buffer) and MDV/HA (containing the active principle in phosphate buffer plus a 0.05% high M.W. Hyaluronic Acid). Precorneal Residence Time (PRT) of MDV and MDV/HA were measured, after instillation of the drugs in the conjunctival sac of rabbits, by means of UV Reflectance Spectroscopy technique. In order to evaluate the influence of mucoadhesivity, experiments were conducted both in normal rabbits and rabbits pre-treated topically with N-acetylcysteine, a well known mucolytic agent.

Results: : Hydrocortisone in the Hyaluronic Acid formulation (MDV/HA, viscosity=2.46 mPas) showed a PRT of 1825+150 sec in the normal rabbits and 550+75 sec in the N-acetylcysteine pre-treated animals. Without the HA mucoadhesive polymer, Hydrocortisone in phosphate buffer solution (MDV) had a PRT of 350+50 sec and 275+42 sec respectively. HA increased by 5.2 times the PRT of HY formulation. The mucoadhesivity was responsible for 86% of the increase of PRT while viscosity for 14%.

Conclusions: : The highly mucoadhesive Hydrocortisone/Hyaluronic Acid topical association, showing a surprisingly long Precorneal Residence Time, could find application in the treatment of ocular surface irritation/inflammation when a soft steroid is suggested.

Keywords: corticosteroids • anterior segment • inflammation 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.