April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Effects Of Chrysene On Human Arpe-19 Cells
Author Affiliations & Notes
  • Rafael Migon
    Gavin Herbert Eye Institute, Univ of California, Irvine, Irvine, California
  • Saffar Mansoor
    Gavin Herbert Eye Institute, Univ of California, Irvine, Irvine, California
    School of Chemical & Biomolecular Engineering, Georgia Institute of Technology. Atlanta, GA, Atlanta, Georgia
  • Baruch D. Kuppermann
    Gavin Herbert Eye Institute, Univ of California, Irvine, Irvine, California
  • Maria C. Kenney
    Gavin Herbert Eye Institute, Univ of California, Irvine, Irvine, California
  • Footnotes
    Commercial Relationships  Rafael Migon, None; Saffar Mansoor, None; Baruch D. Kuppermann, None; Maria C. Kenney, None
  • Footnotes
    Support  Supported by the Discovery Eye Foundation, the Henry L. Guenther Foundation, the Iris and B, Gerald Cantor Foundation, Gilbert Foundation, Ko Family Foundation and Research to Prevent Blindness Founda
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4429. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Rafael Migon, Saffar Mansoor, Baruch D. Kuppermann, Maria C. Kenney; Effects Of Chrysene On Human Arpe-19 Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4429.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To evaluate the in vitro toxicity of chrysene on human retinal pigment epithelial cells (ARPE-19).

Methods: : ARPE-19 cells were exposed to different concentrations of Chrysene diluted in DMSO (250µM, 500µM, 750µM and 1000µM). Cells were incubated at 37º C until confluent. Before drug exposure, the cells were incubated for 24 hours in fetal bovine serum free medium to make them relatively non-proliferating. After 24 hours of chrysene exposure, the following assays were performed: trypan blue dye exclusion to measure cell viability (CV), JC-1 to measure changes in mitochondrial membrane potential (ΔΨM), caspase-3/7 as an indicator of apoptotic activity and the 2’, 7’ dichlorodihydrofluorescein diacetate (DCFH-DA) assay to measure accumulation of reactive oxygen species (ROS).

Results: : ARPE-19 cells treated with chrysene mean % CV was 93.65%, 81.10%, 77.55% and 60.05% for 250, 500, 750 and 1000 µM respectively, the later showed significantly reduction in CV% compared to untreated ARPE19 controls. The ΔΨM was decreased in all chrysene concentrations when compared to untreated ARPE 19 controls (14.56±0.167). The mean ΔΨm values were 4.5±0.19, 3.4±0.14, 4.3±0.22 and 3.5±0.5 for Chrysene 250, 500, 750 and 1000µM respectively. ROS activity was significantly increased for all chrysene concentrations when compared to untreated control. The mean fluorescence values were 36717±627 msi, 40097±620 msi, 37044±511 msi and 38040±1743, for Chrysene 250, 500, 750 and 1000µM respectively as compared to untreated controls (18064±1484 msi). Caspase 3/7 activity was significantly increased for all chrysene concentrations. The mean fluorescence values were 6179±316 msi, 6424±334 msi, 8345±551 msi and 11235±539 for Chrysene 250, 500, 750 and 1000 µM respectively as compared to untreated cells (2564±425 msi). Toxicity for the chrysene solvent DMSO was tested for all treatment groups and no statistically significant change was seen.

Conclusions: : Chrysene in all concentrations used in this study significantly decreases retinal cell viability in vitro, induces JC1, ROS, and caspase 3/7 activity.

Keywords: retinal culture • oxidation/oxidative or free radical damage • apoptosis/cell death 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×