April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Synergistic Effects of Vitamin E and Vitamin C Modulation of AP-1 Gene Regulation in the Human Retinal Pigment Epithelium Under Oxidative Stress
Author Affiliations & Notes
  • Jinggang Yin
    Hamilton Eye Institute, University of Tennessee HSC, Memphis, Tennessee
  • Weihong Huo
    Hamilton Eye Institute, University of Tennessee HSC, Memphis, Tennessee
  • J.C. Lang
    Alcon Research Ltd., Ft.Worth, Texas
  • Edward Chaum
    Hamilton Eye Institute, University of Tennessee HSC, Memphis, Tennessee
    Alcon Research Ltd., Ft.Worth, Texas
  • Footnotes
    Commercial Relationships  Jinggang Yin, None; Weihong Huo, None; J.C. Lang, Alcon Research Ltd. (F), AlconResearch Ltd (P); Edward Chaum, Alcon Research Ltd. (F), AlconResearch Ltd (P)
  • Footnotes
    Support  Alcon Research Ltd., Research to Prevent Blindness, RPB, The Plough Foundation
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4430. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jinggang Yin, Weihong Huo, J.C. Lang, Edward Chaum; Synergistic Effects of Vitamin E and Vitamin C Modulation of AP-1 Gene Regulation in the Human Retinal Pigment Epithelium Under Oxidative Stress. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4430.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : We have previously shown that pretreatment with specific doses of vitamin C can protect the RPE by modulating oxidative stress-induced (OS) AP-1 gene expression in ARPE-19 cells. Other antioxidant micronutrients, especially vitamin E, have been shown to confer protective antioxidant effects in non-retinal cell-lines. The purpose of present study was to determine whether vitamin E can synergistically enhance the protective effects of vitamin C against OS using an in vitro biomarker assay.

Methods: : Confluent ARPE-19 cells were cultured for three days in defined media in the presence of vitamin E (50µM) with vitamin C (50, 100, or 200µM), and then treated for 1 hour with 500µM H2O2. RNA was isolated at 0-, 1-, 4-, 8-, and 24-hours after OS and compared to untreated controls at each time point. Transcription factor gene expression, in particular the AP-1 genes (FosB, cFos, and ATF3), EGR1/2, and the antioxidant gene HO-1 was determined by real-time PCR.

Results: : The characteristic OS response seen in AP-1, EGR2, and HO-1 gene expression was reduced in cells pretreated with vitamin E and vitamin C cells. FosB gene expression was reduced by ~91% and 86% (from 38.5-fold to 3.4-fold, and from 12.8-fold to 1.6-fold ) under OS in the presence of 50µM vitamin E and 100µM vitamin C. ATF3 gene expression was reduced ~24% and ~58% at 1-, and 8-hours after OS, respectively, and EGR2 gene expression was reduced ~85% and ~73% at 1-, and 8-hours after OS, respectively. Similarly, HO-1 transcription was reduced more than 70% after 24 hours following OS when pretreated with both vitamin E and vitamin C.

Conclusions: : Vitamin E supplementation in conjunction with vitamin C pretreatment modulates the expression of AP-1 biomarkers, EGR2, and HO-1 in RPE cells under oxidative stress. Vitamin E at 50µM synergistically enhances the protective effects of lower concentrations of vitamin C in the RPE cells by reducing OS-responses that may trigger apoptosis.

Keywords: retinal pigment epithelium • oxidation/oxidative or free radical damage • transcription factors 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×