April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
The Size of Retinal Avascular Areas Measured with Non-invasive RFI and Flourescein Angiography
Author Affiliations & Notes
  • Darin A. Nelson
    Optical Imaging Ltd, Rehovot, Israel
  • Amiram Grinvald
    Neurobiology, Weizmann Institute of Science, Rehovot, Israel
  • Alina Sorkin
    Optical Imaging Ltd, Rehovot, Israel
  • Amit Ruf
    Optical Imaging Ltd, Rehovot, Israel
  • Zvia Burgansky-Eliash
    Ophthalmology, The Edith Wolfson Medical Center, Holon, Israel
  • Adiel Barak
    Ophthalmology, Sourasky Medical Center, Tel aviv, Israel
  • Anat Lowenstein
    Ophthalmology, Sourasky Medical Center, Tel aviv, Israel
  • Footnotes
    Commercial Relationships  Darin A. Nelson, Optical Imaging, Ltd (E); Amiram Grinvald, Optical Imaging, Ltd (I, P); Alina Sorkin, Optical Imaging, Ltd (E); Amit Ruf, Optical Imaging, Ltd (E); Zvia Burgansky-Eliash, Optical Imaging, Ltd (E); Adiel Barak, None; Anat Lowenstein, None
  • Footnotes
    Support  Optical Imaging, Ltd
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4473. doi:
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      Darin A. Nelson, Amiram Grinvald, Alina Sorkin, Amit Ruf, Zvia Burgansky-Eliash, Adiel Barak, Anat Lowenstein; The Size of Retinal Avascular Areas Measured with Non-invasive RFI and Flourescein Angiography. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4473.

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      © ARVO (1962-2015); The Authors (2016-present)

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We used the Retinal Function Imager (RFI, Optical Imaging, Ltd)to image the capillaries network non-invasively. We comparedthe non-invasive measurements of foveal avascular zone (FAZ)and retinal non-perfusion areas to measurements with flouresceinangiography (FA).


Fifteen patients with retinal ischemic diseases were recruited;10 with diabetic retinopathy (DR) and 5 with vein occlusion(VO). Multiple RFI retinal image series were acquired . Followingregistration, pixel values were analyzed to locate blood cellsmotion, thus obtaining non-invasive capillary perfusion maps(nCPM) at high resolution. Patients had also FA imaging, nCPMand FA images were aligned and scale using Inkscape software.FAZ and retinal non-perfusion areas dimensions were measuredusing ImageJ (NIH, USA). Comparison between groups was doneusing student t-test.


Only the differences in FAZ area dimensions in the DR groupwere statistical significant (table, p=0.016).


Using the RFI we measured larger avascular areas compared tothe measurements with FA in patients with DR. This may representunderestimation of non-perfused area due to dye leakage in FAimages. Non-invasive monitoring of the ischemic area dimensionsmay provide an important tool for early diagnosis and follow-up.  


Keywords: diabetic retinopathy • imaging/image analysis: clinical • vascular occlusion/vascular occlusive disease 

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