March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Carboxypeptidase A3 Expression In Intraepithelial Mast Cells Of Chronic Allergic Conjunctivitis
Author Affiliations & Notes
  • Yukiko Miyagawa
    Juntendo univ., Bunkyo-ku, Tokyo, Japan
  • Akira Matsuda
    Juntendo univ., Bunkyo-ku, Tokyo, Japan
  • Nobuyuki Ebihara
    Juntendo univ., Bunkyo-ku, Tokyo, Japan
  • Toshinari Funaki
    Juntendo univ., Bunkyo-ku, Tokyo, Japan
  • Kanji Hori
    Juntendo univ., Bunkyo-ku, Tokyo, Japan
  • Norihiko Yokoi
    Kyoto Prefectural Univ., Kyoto, Japan
  • Akira Murakami
    Juntendo univ., Bunkyo-ku, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Yukiko Miyagawa, None; Akira Matsuda, None; Nobuyuki Ebihara, None; Toshinari Funaki, None; Kanji Hori, None; Norihiko Yokoi, None; Akira Murakami, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4001. doi:
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      Yukiko Miyagawa, Akira Matsuda, Nobuyuki Ebihara, Toshinari Funaki, Kanji Hori, Norihiko Yokoi, Akira Murakami; Carboxypeptidase A3 Expression In Intraepithelial Mast Cells Of Chronic Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4001.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Carboxypeptidase A3 (CPA3) is a protease known to be expressed in mast cells. Preferential CPA3 expression in intraepithelial mast cells in atopic asthma was reported previously. We investigated the expression of CPA3 in the giant papillae obtained from vernal keratoconjunctivitis (VKC) and atopic keratoconjunctivitis (AKC) patients.

Methods: : 10 giant papillae were resected for therapeutic purpose and frozen sections were prepared as previously described. (IOVS.50:2871-2877) Anti-CPA3/anti-tryptase or anti-CPA3/anti-chymase double immnunohistochemical staining was carried out by indirect immunofluorescent method.

Results: : CPA3-tryptase double immunostaining showed that CPA3 positive staining was observed in the intraepithelial tryptase positive mast cells. CPA3-chymase double immunostaining showed both CPA3+ chymase- mast cells and CPA3+ chymase+ mast cells in the conjunctival epithelium of the giant papillae samples. CPA3+ chymase+ mast cells tend to be located at near the epithelial-stromal borders.

Conclusions: : Preferential CPA3 expression in the intraepithelial mast cells of giant papillae was observed. MCT mast cells are predominant mast cell type for CPA3 expression. CPA3 expression may relate to mast cell activation or maturation.

Keywords: conjunctivitis 

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