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Satoshi Kinoshita, Satoru Kase, Junichi Fukuhara, Zhenyu Dong, Ryo Ando, Anton Lennikov, Atsuhiro Kanda, Kousuke Noda, Susumu Ishida; Expression of Vascular Endothelial Growth Factor in Human Conjunctival Lymphoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4006. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Extranodal marginal zone B-cell lymphoma (EMZL) of the conjunctiva is a common tumor in ocular adnexal lymphomas in human; however, the mechanism underlying the tumor growth and angiogenesis has yet to be elucidated. In this study, we examined the expression of vascular endothelial growth factor (VEGF), known to be a tumor growth factor and an angiogenic factor, in human conjunctival EMZL.
Seven conjunctival EMZLs and a reactive lymphoid hyperplasia (RLH), surgically removed, were examined in this study. Formalin-fixed, paraffin-embedded tissue sections were processed for hematoxylin & eosin staining, and immunohistochemistry with antibodies against VEGF and CD20, a B-cell marker. Colocalization of VEGF with CD20 was evaluated by double-staining immunohistochemistry. Monoclonal immunoglobulin heavy chain (IgH) gene rearrangement was determined by Southern blotting in DNA extracted from these tissues.
In conjunctival EMZLs, CD20-positive atypical lymphoid cells with monoclonal IgH gene rearrangement diffusely infiltrated the conjunctival stroma. In RLH, CD20-positive normal-appearing lymphocytes without monoclonality infiltrated the subepithelium. Immunoreactivity for VEGF was detected in the cytoplasm of CD20-positive cells, in which VEGF-immunopositive rate was 56 % and 13 % in conjunctival EMZL and RLH, respectively.
These results suggest that VEGF is predominantly expressed in human conjunctival lymphoma cells, which may contribute to tumor growth and angiogenesis.
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