March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Expression of Vascular Endothelial Growth Factor in Human Conjunctival Lymphoma
Author Affiliations & Notes
  • Satoshi Kinoshita
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Satoru Kase
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Junichi Fukuhara
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Zhenyu Dong
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Ryo Ando
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Anton Lennikov
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Atsuhiro Kanda
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Kousuke Noda
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Susumu Ishida
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Footnotes
    Commercial Relationships  Satoshi Kinoshita, None; Satoru Kase, None; Junichi Fukuhara, None; Zhenyu Dong, None; Ryo Ando, None; Anton Lennikov, None; Atsuhiro Kanda, None; Kousuke Noda, None; Susumu Ishida, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4006. doi:https://doi.org/
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      Satoshi Kinoshita, Satoru Kase, Junichi Fukuhara, Zhenyu Dong, Ryo Ando, Anton Lennikov, Atsuhiro Kanda, Kousuke Noda, Susumu Ishida; Expression of Vascular Endothelial Growth Factor in Human Conjunctival Lymphoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4006. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Extranodal marginal zone B-cell lymphoma (EMZL) of the conjunctiva is a common tumor in ocular adnexal lymphomas in human; however, the mechanism underlying the tumor growth and angiogenesis has yet to be elucidated. In this study, we examined the expression of vascular endothelial growth factor (VEGF), known to be a tumor growth factor and an angiogenic factor, in human conjunctival EMZL.

Methods: : Seven conjunctival EMZLs and a reactive lymphoid hyperplasia (RLH), surgically removed, were examined in this study. Formalin-fixed, paraffin-embedded tissue sections were processed for hematoxylin & eosin staining, and immunohistochemistry with antibodies against VEGF and CD20, a B-cell marker. Colocalization of VEGF with CD20 was evaluated by double-staining immunohistochemistry. Monoclonal immunoglobulin heavy chain (IgH) gene rearrangement was determined by Southern blotting in DNA extracted from these tissues.

Results: : In conjunctival EMZLs, CD20-positive atypical lymphoid cells with monoclonal IgH gene rearrangement diffusely infiltrated the conjunctival stroma. In RLH, CD20-positive normal-appearing lymphocytes without monoclonality infiltrated the subepithelium. Immunoreactivity for VEGF was detected in the cytoplasm of CD20-positive cells, in which VEGF-immunopositive rate was 56 % and 13 % in conjunctival EMZL and RLH, respectively.

Conclusions: : These results suggest that VEGF is predominantly expressed in human conjunctival lymphoma cells, which may contribute to tumor growth and angiogenesis.

Keywords: conjunctiva • tumors • immunohistochemistry 
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