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Siv Johnsen-Soriano, Raul Martinez-Belda, Cristina Peris-Martinez, Emma Arnal, Diana Martinez, Javier Romero; Expression Of Cell Proliferation And Oxidative Stress Biomarkers In Pterygium And Normal Conjunctiva. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4020.
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To analyze the expression of oxidative stress and cell-proliferation molecules in pterygium tissue.
18 pterygium were surgically removed using the free superior bulbar conjuctiva FLAP procedure and 15 normal bulbar conjunctivas were also obtained. The research reported was conducted in compliance with "Declaration of Helsinki". Formalin-fixed, paraffin-wax-embedded tissues were analyzed by immunohistochemistry with Ki-67 (a proliferating cell marker) and HNE (Hydroxynonenal; an oxidative stress marker) antibodies.
Our study revealed that the positive rate of Ki-67 positive cell density significantly increased in the pterygium samples (70,8 ± 5,0 Ki-67 positive cells/ total cells) compared to the normal conjunctiva samples (60,9 ± 2,7 Ki-67 positive cells/ total cells). HNE positive cell number was significantly higher in the pterygium samples (75,0± 3.5 HNE positive cells/ total cells) than in the conjunctiva samples (66,9 ± 5.2 HNE positive cells/ total cells).
The finding of higher levels of cellular proliferation and oxidative stress in pterygium confirmed that both oxidative stress and proliferation are known to play an important role in human pterygium pathogenesis.
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