March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Expression Profile Of RhoA And Rho Kinase In The Human Corneal Epithelium And Endothelium
Author Affiliations & Notes
  • Noel Rosado-Adames
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Brian Goldhagen
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Landon C. Meekins
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Alan Proia
    Pathology, Duke University Medical Center, Durham, North Carolina
  • David L. Epstein
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Vasantha Rao
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Natalie A. Afshari
    Ophthalmology, Duke University Eye Center, Durham, North Carolina
  • Footnotes
    Commercial Relationships  Noel Rosado-Adames, None; Brian Goldhagen, None; Landon C. Meekins, None; Alan Proia, None; David L. Epstein, None; Vasantha Rao, None; Natalie A. Afshari, None
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4199. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Noel Rosado-Adames, Brian Goldhagen, Landon C. Meekins, Alan Proia, David L. Epstein, Vasantha Rao, Natalie A. Afshari; Expression Profile Of RhoA And Rho Kinase In The Human Corneal Epithelium And Endothelium. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4199.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : To explore the potential involvement of RhoA and Rho kinase in corneal physiology and tissue regeneration, we evaluated the distribution profile of RhoA and Rho kinase (ROCK-1 and ROCK-2) in human corneal epithelial and endothelial cells.

Methods: : Paraffin-embedded postmortem eyes from six human subjects (age 73-90 years) with no known corneal pathology were stained immunohistochemically for RhoA, ROCK-1, and ROCK-2 proteins with respective polyclonal antibodies. The distribution and intensity of the immunostaining in the corneal endothelium and epithelium was assessed using light microscopy.

Results: : All six human eyes evaluated showed a positive staining for RhoA, ROCK-1, and ROCK-2 in the corneal epithelium and endothelium. No significant difference was found in the relative intensity of the staining of each of these proteins in human corneal epithelial and endothelial cells. The intensity of immunostaining of RhoA, ROCK-1 and ROCK-2 in the corneal epithelium was found to increase gradiently from its basal to apical layer.

Conclusions: : This study demonstrates the expression profile of RhoA, ROCK-1 and ROCK-2 in human corneal endothelial and epithelial cells. These molecules may become therapeutic targets for certain corneal disorders in the future.

Keywords: cornea: endothelium • cornea: epithelium • microscopy: light/fluorescence/immunohistochemistry 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×