April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Corneal Graft Rejection In The Setting Of Previous Hsv-1 Infection
Author Affiliations & Notes
  • Carlos A. Medina-Mendez
    Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
  • Jared E. Knickelbein
    Ophthalmology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania
  • Guillermo Amescua
    Ophthalmology, University of Miami Bascom Palmer Eye Insititute, MIami, Pennsylvania
  • John V. Forrester
    Div of Applied Medicine, Imm & Inf, University of Aberdeen, Aberdeen, United Kingdom
  • Robert L. Hendricks
    Ophthalmology, Univ of Pittsburgh Eye & Ear Inst, Pittsburgh, Pennsylvania
  • Footnotes
    Commercial Relationships  Carlos A. Medina-Mendez, None; Jared E. Knickelbein, None; Guillermo Amescua, None; John V. Forrester, None; Robert L. Hendricks, None
  • Footnotes
    Support  NIH RO1-EY05945, NIH RO1-EY10359, NIH P-30EY08098, Research to Prevent Blindness Inc. Eye and Ear Foundation of Pittsburgh
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4761. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Carlos A. Medina-Mendez, Jared E. Knickelbein, Guillermo Amescua, John V. Forrester, Robert L. Hendricks; Corneal Graft Rejection In The Setting Of Previous Hsv-1 Infection. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4761. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Corneal grafts placed in patients with a history of ocular HSV-1 infection reject more quickly and often than grafts placed for non-inflammatory indications. The purpose of this study was to investigate the mechanism of corneal graft rejection in the high-risk setting of previous corneal HSV-1 infection.

Methods: : CD4 KO Balb/c mice were infected with HSV-1 via the cornea for 10 days prior to grafting with either WT Balb/c (syngraft) or C57BL/6 (allograft) corneal donor buttons. CD4 KO Balb/c hosts received 2x106 naïve Balb/c CD4 T cells at time of grafting or not. Graft rejection was assessed by clinical biomicroscopy and scored based on corneal opacity. HSV-1 reactivation was assessed by clinical examination for recurrent periocular vesicular lesions and measurement of HSV-1 genome copy number in trigeminal ganglia of transplanted mice.

Results: : HSV-1 infection of CD4 KO Balb/c mice prior to allograft resulted in 100% rejection of grafts by 14 days post-transplant in mice that received CD4 T cell transfer. A portion of these mice demonstrated periocular vesicular lesions. Conversely, only ~10% of allografts rejected in HSV-1-infected mice that did not receive CD4 T cells. HSV-1 infection of CD4 KO Balb/c mice prior to syngeneic corneal transplantation resulted in ~30% graft rejection when CD4 T cells were transferred at the time of transplant. No rejection was seen in allografts placed onto non-infected CD4 KO Balb/c mice that received CD4 T cells. HSV-1 genome copy number was higher in trigeminal ganglia of HSV-infected CD4 KO Balb/c mice who rejected their allografts (i.e. received CD4 T cells) than those who did not (i.e. no CD4 T cells). No difference in HSV-1 genome copy number was observed in HSV-infected CD4 KO mice who received syngeneic grafts with or without CD4 T cell transfer.

Conclusions: : HSV-1 infection accelerates corneal graft rejection. CD 4 T cells are required for corneal graft rejection in the setting of previous HSV-1 infection. Allograft rejection in the setting of previous HSV-1 infection was associated with a higher number HSV-1 genome copies. Therefore, HSV-1 reactivation following corneal transplant likely contributes to the more rapid rejection.

Keywords: herpes simplex virus • keratitis • transplantation 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×