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Takeshi Yoshida, Mizuki Kitamura, Shikun He, Christine Spee, Stephen J. Ryan, David R. Hinton; Histone Acetylation is Regulated by αB-crystallin in Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4787.
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αB-crystallin is known as a member of the small heat shock protein family and functions in apoptosis inhibition and cellular protection. The aim of the present study is to investigate whether αB-crystallin is involved in the regulation of histone acetylation modifications in retina.
αB-crystallin-/- mice (129S6/SvEvTac background) and 129S6/SvEvTac wild-type mice were used for the study. Cryostat retina sections of snap-frozen mouse eyes were obtained from these mice. The expression of acetylated histone H3 and histone deacetylase 1(HDAC1) was investigated by immunohistochemistry.
The expression of acetylated histone H3 in ganglion cells and retinal pigment epithelial cells (RPE) in αB-crystallin-/- mice was lower than the one in wild-type, however, the expression of HDAC 1 was higher in ganglion cells and RPE in αB-crystallin-/- mice .
Our data show that αB-crystallin play a role in the regulation of the expression of acetylated histone H3 and HDAC 1 in retina. These results suggest that the physiological function of αB-crystallin may reside in its association with histone acetylation.
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