April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Histone Acetylation is Regulated by αB-crystallin in Retina
Takeshi Yoshida; Mizuki Kitamura; Shikun He; Christine Spee; Stephen J. Ryan; David R. Hinton
Author Affiliations & Notes
  • Takeshi Yoshida
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Ophthalmology,
    Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Mizuki Kitamura
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Ophthalmology,
    Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Shikun He
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Pathology,
    Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Christine Spee
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Ophthalmology,
    Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Stephen J. Ryan
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Ophthalmology,
    Keck School of Medicine of the University of Southern California, Los Angeles, California
  • David R. Hinton
    Ophthalmology, Doheny Eye Institute, Los Angeles, California
    Pathology,
    Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Footnotes
    Commercial Relationships  Takeshi Yoshida, None; Mizuki Kitamura, None; Shikun He, None; Christine Spee, None; Stephen J. Ryan, None; David R. Hinton, None
  • Footnotes
    Support  NIH grants EY01545, EY03040 & grants from RPB & the Arnold & Mabel Beckman foundation
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4787. doi:
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      Takeshi Yoshida, Mizuki Kitamura, Shikun He, Christine Spee, Stephen J. Ryan, David R. Hinton; Histone Acetylation is Regulated by αB-crystallin in Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4787.

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Abstract

Purpose: : αB-crystallin is known as a member of the small heat shock protein family and functions in apoptosis inhibition and cellular protection. The aim of the present study is to investigate whether αB-crystallin is involved in the regulation of histone acetylation modifications in retina.

Methods: : αB-crystallin-/- mice (129S6/SvEvTac background) and 129S6/SvEvTac wild-type mice were used for the study. Cryostat retina sections of snap-frozen mouse eyes were obtained from these mice. The expression of acetylated histone H3 and histone deacetylase 1(HDAC1) was investigated by immunohistochemistry.

Results: : The expression of acetylated histone H3 in ganglion cells and retinal pigment epithelial cells (RPE) in αB-crystallin-/- mice was lower than the one in wild-type, however, the expression of HDAC 1 was higher in ganglion cells and RPE in αB-crystallin-/- mice .

Conclusions: : Our data show that αB-crystallin play a role in the regulation of the expression of acetylated histone H3 and HDAC 1 in retina. These results suggest that the physiological function of αB-crystallin may reside in its association with histone acetylation.

Keywords: retina • chaperones • crystallins 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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