April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Mast Cell-conjunctival Epithelial Cell Interaction Induces Macrophage Chemoattractant Peptide-1 (mcp-1) Expression In Chronic Allergic Conjunctivitis
Satoshi Iwamoto; Akira Matsuda; Nobuyuki Ebihara; Toshinari Funaki; Kanji Hori; Tsutomu Inatomi; Satoshi Kawasaki; Norihiko Yokoi; Yoshimichi Okayama; Akira Murakami
Author Affiliations & Notes
  • Satoshi Iwamoto
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Akira Matsuda
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Nobuyuki Ebihara
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Toshinari Funaki
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Kanji Hori
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Tsutomu Inatomi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Satoshi Kawasaki
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Norihiko Yokoi
    Ophthalmology, Kyoto Prefectural Univ of Med, Kyoto, Japan
  • Yoshimichi Okayama
    Division of Molecular Cell Immunology and Allergology, Nihon University School of Medicine, Tokyo, Japan
  • Akira Murakami
    Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Satoshi Iwamoto, None; Akira Matsuda, None; Nobuyuki Ebihara, None; Toshinari Funaki, None; Kanji Hori, None; Tsutomu Inatomi, None; Satoshi Kawasaki, None; Norihiko Yokoi, None; Yoshimichi Okayama, None; Akira Murakami, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4790. doi:
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      Satoshi Iwamoto, Akira Matsuda, Nobuyuki Ebihara, Toshinari Funaki, Kanji Hori, Tsutomu Inatomi, Satoshi Kawasaki, Norihiko Yokoi, Yoshimichi Okayama, Akira Murakami; Mast Cell-conjunctival Epithelial Cell Interaction Induces Macrophage Chemoattractant Peptide-1 (mcp-1) Expression In Chronic Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4790.

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Abstract

Purpose: : Intraepithelial mast cells are observed in the giant papillae tissue samples obtained from atopic keratoconjunctivits(AVC)/vernal keratoconunctivitis(VKC) patients. In this study, we examined the roles of interaction between the conjunctival epithelial cells (CE) and mast cells.

Methods: : Giant papillae tissue samples (n=10) were resected for therapeutic purpose, and subjected to histological analysis with informed consents. Ultrastrucutral and immunohistochemical analysis was carried out. Human peripheral blood derived mast cell-CE interaction was investigated using co-culture model. Protein array analysis and realtime PCR was carried out to test the interaction. Finally, recombinant MCP-1 (10ng/ml) was reacted with the cultured human mast cells.

Results: : Ultrastrucutural analysis of giant papillae showed peacemeal degranulation of mast cells within the CE. Protein array and realtime PCR analysis showed MCP-1 protein/mRNA expression was induced by mast cell-CE coculture model. Upregulation of MCP-1 mRNA was observed in mast cells, whereas in vivo MCP-1 expression was observed at the CE and vascular endothelium of the giant papillae by immunohistochemistry. Ultrastrucutural analysis also showed that rMCP-1 could induce peacemeal degranulation in the mast cells.

Conclusions: : Mast cell-CE interaction induces MCP-1 expression, and results in slow persistent release of proinflammatory mediators from mast cells by peacemeal degranulation.

Keywords: conjunctivitis • immunomodulation/immunoregulation • cytokines/chemokines 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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