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Satoshi Iwamoto, Akira Matsuda, Nobuyuki Ebihara, Toshinari Funaki, Kanji Hori, Tsutomu Inatomi, Satoshi Kawasaki, Norihiko Yokoi, Yoshimichi Okayama, Akira Murakami; Mast Cell-conjunctival Epithelial Cell Interaction Induces Macrophage Chemoattractant Peptide-1 (mcp-1) Expression In Chronic Allergic Conjunctivitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4790.
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© ARVO (1962-2015); The Authors (2016-present)
Intraepithelial mast cells are observed in the giant papillae tissue samples obtained from atopic keratoconjunctivits(AVC)/vernal keratoconunctivitis(VKC) patients. In this study, we examined the roles of interaction between the conjunctival epithelial cells (CE) and mast cells.
Giant papillae tissue samples (n=10) were resected for therapeutic purpose, and subjected to histological analysis with informed consents. Ultrastrucutral and immunohistochemical analysis was carried out. Human peripheral blood derived mast cell-CE interaction was investigated using co-culture model. Protein array analysis and realtime PCR was carried out to test the interaction. Finally, recombinant MCP-1 (10ng/ml) was reacted with the cultured human mast cells.
Ultrastrucutural analysis of giant papillae showed peacemeal degranulation of mast cells within the CE. Protein array and realtime PCR analysis showed MCP-1 protein/mRNA expression was induced by mast cell-CE coculture model. Upregulation of MCP-1 mRNA was observed in mast cells, whereas in vivo MCP-1 expression was observed at the CE and vascular endothelium of the giant papillae by immunohistochemistry. Ultrastrucutural analysis also showed that rMCP-1 could induce peacemeal degranulation in the mast cells.
Mast cell-CE interaction induces MCP-1 expression, and results in slow persistent release of proinflammatory mediators from mast cells by peacemeal degranulation.
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