April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Gold Nanoparticle Inhibits Retinal Neovascularization via Suppression of VEGFR-2 Activation
Author Affiliations & Notes
  • Jeong Hun Kim
    Ophthalmology-Coll of Med, Seoul National Univ Hospital, Seoul, Republic of Korea
  • Jin Hyoung Kim
    Ophthalmology-Coll of Med, Seoul National Univ Hospital, Seoul, Republic of Korea
  • Myung Hun Kim
    Chemistry, Yonsei University, Seoul, Republic of Korea
  • Tae Geol Lee
    NanoBio Fusion Research Center, Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea
  • Young Suk Yu
    Ophthalmology-Coll of Med, Seoul National Univ Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Jeong Hun Kim, None; Jin Hyoung Kim, None; Myung Hun Kim, None; Tae Geol Lee, None; Young Suk Yu, None
  • Footnotes
    Support  Bio-Signal Analysis Technology Innovation Program (2009-0090895) of MEST/NRF, Korea and Mid-Career Researcher Program (07-2010-042-0) of MEST/NRF, Korea
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 4829. doi:
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    • Get Citation

      Jeong Hun Kim, Jin Hyoung Kim, Myung Hun Kim, Tae Geol Lee, Young Suk Yu; Gold Nanoparticle Inhibits Retinal Neovascularization via Suppression of VEGFR-2 Activation. Invest. Ophthalmol. Vis. Sci. 2011;52(14):4829.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : This study was to investigate whether gold nanoparticle (GNP) could inhibit retinal neovascularization in the animal model of ROP.

Methods: : Anti-angiogenic activity of GNP was evaluated by vascular endothelial growth factor (VEGF)-induced proliferation, migration and in vitro tube formation assay of human retinal microvascular endothelial cells (HRMECs). Also, inhibition of VEGFR-2 phosphorylation by decursin was measured by Western blot analysis. After intravitreal injection of decursin in the mouse model of ROP, retinal neovascularization was examined by fluorescence angiography and vessel counting in cross sections. The toxicity of decursin was evaluated through MTT assay in HRMECs as well as histological examination.

Results: : Intravitreal injection of GNP significantly inhibited retinal neovascularization in the mouse model of ROP. In addition, GNP effectively suppressed VEGF-induced in vitro angiogenesis of retinal microvascular endothelial cells including proliferation, migration and capillary-like networks formation. GNP blocked VEGF-induced auto-phosphorylation of VEGFR-2 to inhibit consequently ERK-1/2 activation. GNP never affected on the cellular viability of retinal microvascular endothelial cells and induced no retinal toxicity.

Conclusions: : GNP could be a potent inhibitor to retinal neovascularization without retinal toxicity. Furthermore, GNP could be extensively applied to variable vaso-proliferative retinopathies mediated by VEGF.

Keywords: retinal neovascularization • vascular endothelial growth factor 
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