April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Tissue Remodelization After Ocular Surface Reconstruction With Intact Or Denuded Amniotic Membrane
Jing Jie; Jie Yang; Hui He; Hua Q. Xiao; Zhi Y. Li; Jing Y. Chen; Nuo Dong; Hu P. Wu; Zu G. Liu; Wei Li
Author Affiliations & Notes
  • Jing Jie
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Jie Yang
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Hui He
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Hua Q. Xiao
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Zhi Y. Li
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Jing Y. Chen
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Nuo Dong
    Ocular Surface Department, Xiamen Eye Hospital, Xiamen, China
  • Hu P. Wu
    Ocular Surface Department, Xiamen Eye Hospital, Xiamen, China
  • Zu G. Liu
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Wei Li
    Eye Institute, Xiamen Univ Medical College, Xiamen, China
  • Footnotes
    Commercial Relationships  Jing Jie, None; Jie Yang, None; Hui He, None; Hua Q. Xiao, None; Zhi Y. Li, None; Jing Y. Chen, None; Nuo Dong, None; Hu P. Wu, None; Zu G. Liu, None; Wei Li, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5163. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Tissue Remodelization After Ocular Surface Reconstruction With Intact Or Denuded Amniotic Membrane
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      Jing Jie, Jie Yang, Hui He, Hua Q. Xiao, Zhi Y. Li, Jing Y. Chen, Nuo Dong, Hu P. Wu, Zu G. Liu, Wei Li; Tissue Remodelization After Ocular Surface Reconstruction With Intact Or Denuded Amniotic Membrane. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5163.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Purpose: : Intact amniotic membrane (iAM), which contains amniotic epithelial cells, was widely used as permanent graft in ocular surface reconstruction surgery. Previous studies showed that corneal epithelial cells cultured on iAM exhibited different biological characteristics compared with those cultured on denuded amniotic membrane (dAM). However, there was no study investigating the efficacy of dAM on ocular surface reconstruction and the tissue remodelization after AM transplantation.

Methods: : Eighteen New Zealand rabbits were performed partial limbal and conjunctival tissue removal (5×5mm in size) in both eyes. The identical size of cryo-preserved human dAM and iAM were then transplanted in hibateral eyes respectively. The epithelial growth on the amniotic membrane was evaluated by fluorescent staining. The expression of basement membrane components type VII collagen and Laminin 5, epithelial differentiation markers such as K19 and K12 cytokeratins, and infiltration of macrophages were investigated by immunostaining. PAS staining was performed to determine goblet cell differentiation. Clinically, twenty pterygium patients were performed with pterygium excision and AM transplantation, 10 of them were transplanted with iAM, while other 10 with dAM. The epithelial growth on the amniotic membrane was also evaluated by fluorescent staining.

Results: : The ocular surface was successfully reconstructed 2 weeks after AM transplantation both in rabbit model and pterygium patients. The growth rate of limbal and conjunctival epithelium was significantly faster on dAM than that on iAM both in rabbit and human. H&E staining of rabbit corneal and conjunctival tissue after AM transplantation showed prominent stratification of epithelium on dAM. PAS staining showed more goblet cells on dAM at 2 weeks after surgery. Basement membrane components collagen VII and Laminin 5 were degraded in dAM within 2 weeks post-surgery, while still remained in iAM. There was less macrophage infiltration in dAM transplantation group. K12 and K19 expression was present in epithelial cells spread on iAM or dAM, and showed no significant difference.

Conclusions: : dAM facilitates epithelial repopulation and goblet cell differentiation during ocular surface reconstruction.

Keywords: wound healing • conjunctiva 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept