March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Lacking Trpv1 Ion Channel Receptor Suppresses Cytokines And Substance P In An Epithelium-debrided Cornea In Mice
Author Affiliations & Notes
  • Takayoshi Sumioka
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Yuka Okada
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Peter S. Reinach
    Biological Sciences, SUNY College of Optometry, New York, New York
  • Norihito Fujita
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Masayasu Miyajima
    Laboratory Animal Center,
    Wakayama Medical University, Wakayama, Japan
  • Shizuya Saika
    Ophthalmology,
    Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships  Takayoshi Sumioka, None; Yuka Okada, None; Peter S. Reinach, None; Norihito Fujita, None; Masayasu Miyajima, None; Shizuya Saika, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4746. doi:
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      Takayoshi Sumioka, Yuka Okada, Peter S. Reinach, Norihito Fujita, Masayasu Miyajima, Shizuya Saika; Lacking Trpv1 Ion Channel Receptor Suppresses Cytokines And Substance P In An Epithelium-debrided Cornea In Mice. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4746.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the effects of lacking transient receptor potential cation channel subfamily V member 1 (TRPV1) receptor on expression of transforming growth factor β1 (TGFβ1), substance P and interleukin-6 (IL-6), all of which are involved in epithelial healing in cornea in mice. Healing of the epithelial defect was also assayed. Substance P is known to be expressed in sensory nerve fibers in cornea.

Methods: : Corneal epithelial wounds were made in TRPV1 -/- (KO, n = 35) and wild type (WT) mice (n = 35) with 2mm trephine. Healing corneas were processed for total RNA extraction and real-time RT-PCR for TGFβ1, substance P and IL-6 at 6, 12 and 18 hrs post-debridement. Healing pattern of the epithelial defect was also evaluated by the measurement of the remaining defect and histological detection of BrdU-labeled epithelial cells.

Results: : Expression of mRNAs of Substance P and IL-6, but not TGFβ1, was significantly less in a KO cornea as compared with a WT cornea at 12 hrs. The loss of TRPV1 receptor impaired resurfacing of the defected corneal epithelium and also suppressed cell proliferation in healing epithelium 24 and 36 hours.

Conclusions: : Lacking TRPV1 ion channel receptor suppressed mRNA expression of substance P and IL-6, as well as impaired healing of corneal epithelium in mice.

Keywords: cornea: epithelium • wound healing • cytokines/chemokines 
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