March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Retinal Function changes in IDE KO mice
Author Affiliations & Notes
  • Kumar Sambamurti
    Neurosciences,
    Medical University of South Carolina, Charleston, South Carolina
  • Eliza Barnwell
    Neurosciences,
    Medical University of South Carolina, Charleston, South Carolina
  • Beth Coughlin
    Neurosciences,
    Medical University of South Carolina, Charleston, South Carolina
  • Baerbel Rohrer
    Ophthalmology, Med Univ of South Carolina, Charleston, South Carolina
  • Jeffrey Crosson
    Neurosciences,
    Medical University of South Carolina, Charleston, South Carolina
  • Craig E. Crosson
    Ophthalmology, Medical Univ of South Carolina, Charleston, South Carolina
  • Zsolt Ablonczy
    Ophthalmology,
    Medical University of South Carolina, Charleston, South Carolina
  • Annamalai Prakasam
    Neurosciences,
    Medical University of South Carolina, Charleston, South Carolina
  • Padmaraju Vasudevaraju
    Neurosciences,
    Medical University of South Carolina, Charleston, South Carolina
  • Footnotes
    Commercial Relationships  Kumar Sambamurti, None; Eliza Barnwell, None; Beth Coughlin, None; Baerbel Rohrer, None; Jeffrey Crosson, None; Craig E. Crosson, None; Zsolt Ablonczy, None; Annamalai Prakasam, None; Padmaraju Vasudevaraju, None
  • Footnotes
    Support  AG023055, EY019065, Alzheimer's Association IIRG10-173180
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4750. doi:
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    • Get Citation

      Kumar Sambamurti, Eliza Barnwell, Beth Coughlin, Baerbel Rohrer, Jeffrey Crosson, Craig E. Crosson, Zsolt Ablonczy, Annamalai Prakasam, Padmaraju Vasudevaraju; Retinal Function changes in IDE KO mice. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4750.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Insulin degrading enzyme (insulysin) has been identified as the major amyloid beta protein (AB)- degrading enzyme. Several studies have linked retinal degeneration with amyloid deposition in animal models and it has been reported that amyloid deposits accumulate in the eyes of Age related Macular degeneration and Glaucoma subjects. In pursuit of our goal to understand the role of the Alzheimer’s amyloid protein precursor and its processing pathways on retinal function, we have examined retinal sensitivity in insulysin-KO mice.

Methods: : We have generated insulysin-KO mice expressing human Alzheimer’s amyloid protein precursor (APP) in the C57BL6 background and have determined their effects on scotopic ERGs.

Results: : : Although APP transgenic mice showed a significant increase in sensitivity to light as measured by scotopic ERG, we did not observe a further increase in Insulysin-KO mice expressing APP. Interestingly, we did observe a trend towards an increase in a wave and a reduction in b wave in amyloid beta protein precursor (APP) knockout mice an in a homologue, APLP2-KO mice.

Conclusions: : APP transgenic mice exhibit increased photoreceptor and bipolar cell responses. The data are consistent with several reports showing that Aβ facilitates synaptic activity; it is unlikely that these mice show substantial Aβ accumulation at this young age. We plan to age the animals to see if insulysin KO affects ERG signal. Both APP KO mice and APLP2 KO mice show a trend towards reduction in light sensitivity. If this trend holds up and reaches significance, we may conclude that regions other than AB play a role in increasing light sensitivity, given that the AB sequence is not conserved between the two homologues.

Keywords: genetics • retina • inner retina dysfunction: biochemistry and cell biology 
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