April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Transmittance of the UV Light Through Deepithelized Cadaver Cornea During the Cross-linking Procedure
Author Affiliations & Notes
  • Sidney J. Sousa
    Ophthalmology, Univ of Sao Paulo-Sch of Med, Ribeirao Preto, Brazil
  • Liliane Ventura
    Ophthalmic Instrumentation Lab, EESC – University of Sao Paulo, São Carlos, Brazil
  • Victor Lincoln
    Ophthalmic Instrumentation Lab, EESC – University of Sao Paulo, São Carlos, Brazil
  • Footnotes
    Commercial Relationships  Sidney J. Sousa, None; Liliane Ventura, None; Victor Lincoln, None
  • Footnotes
    Support  FAEPA and FAPESP
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5208. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Sidney J. Sousa, Liliane Ventura, Victor Lincoln; Transmittance of the UV Light Through Deepithelized Cadaver Cornea During the Cross-linking Procedure. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5208.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose:
 

To study the transmittance of the UV light through deepithelized cadaver cornea during the cross-linking procedure.

 
Methods:
 

The study included 20 human corneas preserved in Optisol-GS for 3 to 11 days (mean: 6 days). The corneas were washed with saline and laid on top of the lid of a Chiron Ophthalmics corneal storage chamber. About 10cm under this lid a 600 µm core diameter optical fiber connected to a spectrometer (USB 2000 Ocean Optics) received the UV transmitted through the mounting. After the complete deepithelization, one drop of riboflavin 0.1%, 400 mOsm, was instilled on the cornea, every 5 minutes, totaling 12 instillations. The UV treatment (365±5 nm, 3 mW/cm2, 1.51 mW, 5.405 J/cm2, 8mm spot, 45mm distance) started only after the 7th instillation. All measurements were collected 4 minutes after each instillation. The average time taken to collect each spectrum was 2 seconds. The thickness of the naked corneas ranged from 464 to 657µm (mean: 570 µm).

 
Results:
 

The average transmittance of the cornea with no riboflavin instillation was 62% (95% CI: 59 to 65%); after the 6th drop yet with no irradiation it was 32% (95% CI: 28 to 36). The UV irradiation started and after the 7th, 8th, 8th, 9th, 10th,11th and 12th drop the average transmittance was respectively 30% (95% CI: 26 to 34), 28% (95% CI: 24 to 32), 26% (95% CI: 22 to 30), 24% (95% CI: 20 to 28), 22% (95% CI: 19 to 26) and 21% (95% CI: 17 to 25).

 
Conclusions:
 

The average transmittance in terms units of energy after the 7th, 8th,9th,10th ,11th and 12th drop were respectively 0.915 , 0.841, 0.793, 0.731, 0.687, 0.643 mW/cm2. All these results are above the assumed endothelium safety limit of toxicity of 0.35 mW/cm2.  

 
Keywords: cornea: clinical science • cornea: basic science • optical properties 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×