April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
The Effect of Riboflavin-UVA-Induced Collagen Crosslinking on Intraocular Pressure Measurement
Author Affiliations & Notes
  • Irit Bahar
    Ophthalmology, Rabin Medical Center, Petach Tiqva, Israel
  • Igor Kaiserman
    Ophthalmology, Barzilai Medical Center, Ashkelon, Israel
  • Naama Hammel
    Ophthalmology, Rabin Medical Center, Petach Tiqva, Israel
  • David Zadok
    Ophthalmology, Asaf Harofe Medical Center, Zrifin, Israel
  • Eitan Livny
    Ophthalmology, Rabin Medical Center, Petach Tiqva, Israel
  • Footnotes
    Commercial Relationships  Irit Bahar, None; Igor Kaiserman, None; Naama Hammel, None; David Zadok, None; Eitan Livny, None
  • Footnotes
    Support  Rabin Medical Center grant for young researchers, Martie foundation grant,
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5211. doi:
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      Irit Bahar, Igor Kaiserman, Naama Hammel, David Zadok, Eitan Livny; The Effect of Riboflavin-UVA-Induced Collagen Crosslinking on Intraocular Pressure Measurement. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5211.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Corneal collagen cross-linking (CXL) using riboflavin (vitamin B2) and UVA irradiation has recently been introduced as a novel therapeutic option for the treatment of corneal ectasia, such as keratoconus. An increase in overall stiffness and rigidity in human corneas of up to 330% has been reported following this treatment to investigate whether CXL affects the Tonopen measurments of intraocular pressure. (IOP)

Methods: : CXL with 0.1% riboflavin solution and 30 minutes of UVA radiation was performed on the right eye of 15 New Zealand albino adult rabbits (1.8 to 2.4 kg) (30 eyes). The left eye served as a control. IOP was measured by a pressure transducer system (True IOP) and by the Tonopen hand held device (corneal applanation tonometer) before treatment, at 1 week, 1 months and 3 months following CXL. Reference pressure in the globe was increased by increments of 10 mm Hg from 10 to 40 mm Hg, using an anterior chamber infusion on a stand with variable height, and tonopen IOP measurments were recorded for each reference pressure, in both eyes.

Results: : Before CXL, tonopen readings were similar between the two eyes.Tonopen underestimated the true IOP in all cases. Following CXL treatment, IOP measurments were significantly higher in treated eye, at all time interval. (0.009<P<0.03) The most significant difference between true and measured IOP was noticed at 20 mmHg.

Conclusions: : IOP measurments following CXL are overestimated by the Tonopen, probably due to increased stiffness of the treated cornea.

Keywords: cornea: basic science • cornea: clinical science • intraocular pressure 

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