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Shasha Gao, Tingyu Qin, Carlos F. Ronchi, Kyung-jin Yeum, Allen Taylor, Yizhi Liu, Fu Shang; Lutein and Zeaxanthin Supplementation Reduce H2O2-Induced Protein Oxidation and DNA Damage in Human Lens Epithelial Cells (HLEC). Invest. Ophthalmol. Vis. Sci. 2011;52(14):5292.
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© ARVO (1962-2015); The Authors (2016-present)
Several epidemiological studies indicate that dietary intake of lutein and zeaxanthin is inversely related to the risk for senile cataract. Since oxidative stress is implicated in cataractogenesis, and lutein and zeaxanthin are antioxidants, we hypothesized that lutein and zeaxanthin may reduce the risk for senile cataract by protecting the lens cells from oxidative damage. The objective of the work was to evaluate the protective effects of lutein and zeaxanthin supplementation against oxidative damage in HLEC.
HLEC (SRA 01/04) were pre-incubated with or without lutein (5µM), zeaxanthin (5µM) or α-tocopherol (5µM) for 48 h and then exposed to 100 µM H2O2 for 1 h. Protein carbonyl formation in HLEC was measured by western-blotting analysis following reaction with dinitrophenyl hydrazine. DNA damage was assessed using a sensitive single cell gel electrophoresis technique (comet assay). Cell viability was monitored by MTS assay.
In the absence of H2O2, HLEC had very low levels of oxidized proteins and supplementation with lutein, zeaxanthin or α-tocopherol had no detectable effects on levels of oxidized proteins in the cells. Exposure of HLEC to H2O2 significantly increased levels of oxidized proteins and DNA damage. Pre-incubation with lutein, zeaxanthin or α-tocopherol dramatically reduced the levels of protein carbonyls in HLEC. H2O2 -induced DNA damage was also decreased by pre-incubation with lutein, zeaxanthin or α-tocopherol. The protective effects of lutein, zeaxanthin and α-tocopherol against protein oxidation and DNA damage were comparable. However, supplementation with lutein, zeaxanthin or α-tocopherol did not ameliorate H2O2-induced loss of cell viability.
These data indicate that supplementation with lutein and zeaxanthin protects lens proteins and DNA from oxidative damage and the protective effects are comparable to that of α-tocopherol. The data imply that sufficient intake of lutein and zeaxanthin may reduce the risk for senile cataract via protecting lens protein and DNA from oxidative damage.
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