April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
3d X-ray Imaging Of The Zonular Apparatus In The Baboon (papio Anubis) Eye
Author Affiliations & Notes
  • Jer R. Kuszak
    Ophthalmology, Rush University Medical Center, Chicago, Illinois
  • Adam M. Zysk
    Electrical and Computer Engineering,
    Illinois Institute of Technology, Chicago, Illinois
  • Mark A. Anastasio
    Biomedical Engineering,
    Illinois Institute of Technology, Chicago, Illinois
  • Footnotes
    Commercial Relationships  Jer R. Kuszak, None; Adam M. Zysk, None; Mark A. Anastasio, None
  • Footnotes
    Support  NIH Grant EY06642
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5300. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Jer R. Kuszak, Adam M. Zysk, Mark A. Anastasio; 3d X-ray Imaging Of The Zonular Apparatus In The Baboon (papio Anubis) Eye. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5300.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

X-ray phase-contrast tomography employs the spatial distribution of a sample’s refractive index to augment or replace conventional absorption in x-ray radiography. Planar in-line phase-contrast imaging is a Gabor holography technique that uses a highly coherent x-ray wavefield and significant post-sample propagation distances to generate interference effects at tissue discontinuities. As the in-line imaging derives contrast from refractive index boundaries, the air-tissue interface produces an effective contrast medium. The purpose of this study was to determine if x-ray phase-contrast could be effectively used to examine the 3-dimensional complexity of critical point dried ocular structures (the lens, ciliary bodies and zonules).


Eyes from baboons were surgically removed and placed into fix (2.5% glutaraldehyde in 0.12M sodium from the eye respectively at the limbus and the ora seratta. The intact lens, still suspended by the zonules and the ciliary bodies were imaged with a benchtop in-line phase-contrast tomography system consisting of a microfocus x-ray tube source, a digital x-ray camera with a custom thermoelectric cooling system, a two-axis stage for sample positioning, and a rotation stage for tomographic imaging.


X-ray phase contrast tomography of the critical point dried ocular tissue described above produced a high-resolution image of the lens suspended by a complete, intact zonular apparatus. This enabled us to create accurate 3-D volumetric renderings of critically important ocular structures responsible for accommodation.


X-ray phase-contrast tomography can be effectively used to more fully comprehend how the complex 3-dimensionality of lenses, is altered during function (accommodation) and with age (presbyopia) because the very 3-dimensionality that is to be studied does not need to be compromised when preparing it for analysis as is the case with conventional techniques (thick and thin sectioning for light and transmission electron microscopy).

Keywords: microscopy: electron microscopy • anatomy • ciliary body 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.