March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Ge-25-like Immunoreactivity In The Rat Eye
Author Affiliations & Notes
  • Katrin Lorenz
    Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Mainz, Mainz, Germany
  • Oliver W. Gramlich
    Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Mainz, Mainz, Germany
  • Franz H. Grus
    Experimental Ophthalmology, Department of Ophthalmology, University Medical Center Mainz, Mainz, Germany
  • Daniela Ehrlich
    Department of Psychiatry and Psychotherapy, Laboratory of Psychiatry and Experimental Alzheimers Research, Innsbruck, Austria
  • Christian Humpel
    Department of Psychiatry and Psychotherapy, Laboratory of Psychiatry and Experimental Alzheimers Research, Innsbruck, Austria
  • Josef Troger
    Ophthalmology & Optometry, University of Innsbruck, Innsbruck, Austria
  • Footnotes
    Commercial Relationships  Katrin Lorenz, None; Oliver W. Gramlich, None; Franz H. Grus, None; Daniela Ehrlich, None; Christian Humpel, None; Josef Troger, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 4918. doi:
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    • Get Citation

      Katrin Lorenz, Oliver W. Gramlich, Franz H. Grus, Daniela Ehrlich, Christian Humpel, Josef Troger; Ge-25-like Immunoreactivity In The Rat Eye. Invest. Ophthalmol. Vis. Sci. 2012;53(14):4918.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the presence and distribution of the chromogranin A-derived neuropeptide GE-25 in the rat eye.

Methods: : The molecular form of GE-25 detected by the GE-25 antiserum was evaluated in the rat trigeminal ganglion, retina and remaining tissues of the rat eye by means of western blots and the distribution pattern of GE-25-like immunoreactivity was studied in the rat eye and rat trigeminal ganglion by immunofluorescence.

Results: : One single band of approximately 70 kDa was stained in the trigeminal ganglion and retina which represents the uncleaved intact chromogranin A indicating that the proteolytic processing of chromogranin A to GE-25 is limited in these tissues. Sparse GE-25-like immunoreactive nerve fibers were visualized in the corneal stroma, at the limbus around blood vessels, in the sphincter and dilator muscle and stroma of the iris, in the stroma of the ciliary body and ciliary processes and in the stroma and around blood vessels in the choroid. Furthermore, immunoreactivity was present in the corneal endothelium and in Müller glia in the retina which is atypical for neuropeptides. In the trigeminal ganglion GE-25-like immunoreactivity was found in small to medium-sized ganglion cells with a diameter of 20 to 30 µm.

Conclusions: : GE-25 like immunoreactivity is distinctly distributed throughout the rat eye and the innervation pattern is predominantly characteristic for neuropeptides. The nerve fibers in the rat eye are obviously of sensory origin.

Keywords: anatomy • immunohistochemistry • retina 
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