April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Raman Spectra of Microbes in Synthetic Model Tears
Author Affiliations & Notes
  • Ming-Tse Kuo
    Department of Ophthalmology, Chang Gung Memorial Hospital, Kaohsiung Medical Center, Kaohsiung, Taiwan
    Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
  • Chi-Chang Lin
    Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan
  • Hsien-Chang Chang
    Institute of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
    Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, Taiwan
  • Footnotes
    Commercial Relationships  Ming-Tse Kuo, None; Chi-Chang Lin, None; Hsien-Chang Chang, None
  • Footnotes
    Support  CMRP Grant CMRPG880701, NSC Grant NSC 99-2218-E-029-004, NSC99-2314-B-182A-030-MY3 and NMRP Grant NMRPG896031
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5824. doi:
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    • Get Citation

      Ming-Tse Kuo, Chi-Chang Lin, Hsien-Chang Chang; Raman Spectra of Microbes in Synthetic Model Tears. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5824.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To investigate the Raman spectra of microbes common in keratitis by a synthetic tear model.

Methods: : We applied the confocal Raman microspectroscopy based on the drop coating deposition (DCDR) method on Ti/Au-coated glass slide to sample spectra from synthetic tears (ST), ST mixed with microbes, including Staphylococcus aureus, Pseudomonas aeruginosa, Fusarium solani, and Acanthamoba castellanii. Raman spectra collected by a line-mapping sampling procedure in the different locations of a dried tear drop. After a series of post-spectral processing, spectra were compared by spectral morphology directly and by the results of principle component analysis.

Results: : The characteristic features either from specific Raman shifts or the principle components for further microbial discrimination were shown. The normalized spectra are different among pure STs or STs with microbes. The tear model based on the normalized spectra by using DCDR method was discriminative for microbes common in keratitis.

Conclusions: : The discriminative Raman spectra of microbes in model tears might provide an encouraging prospect on the differential potential of keratitis through tear Raman spectra.

Keywords: cornea: tears/tear film/dry eye • microscopy: confocal/tunneling • detection 

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