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Hiroshi Eguchi, Tatsuro Miyamoto, Yoshinori Mitamura, Fumio Saito, Katsuhide Yamazaki; Efficacy Of Povidone-iodine For Disinfection Of Acanthamoeba Trophozoite. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5827.
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To compare the efficacy of povidone-iodne with commercially available multipurpose solution against Acanthamoeba trophozoite with viewing the morphological change
Acanthamoeba castellanii (ATCC 50514) was incubated in flask with PYG medium at 25 degree C. After decantation of the medium, the bottom of the flask was scraped by cell scraper and rinsed with Page’s amoeba saline(PAS)twice. The suspension of the PAS was centrifuged and the supernatant was controlled to 1x104~1x105 trophozoites/ml as a source of inoculum. The inoculum was cultured stationary for two hours at 25 degree C in 12 wells plate. Examining the plate with phase-contrast microscope, morphological changes of trophozoites were recorded on a digital video after injection of disinfectants onto each well directly. Seventeen hours later, PYG medium was added in each well to confirm whether trophozoites have a regrowth capacity. Two major brands(MPS A, MPS B)were recruited in this study.
Our study allowed viewing Acanthamoeba trophozoites’ morphological changes with time. The motion of trophozoites stopped quickly after injection of povidone-iodine, and effusion of cytoplasm occurred in most of the trophozoites about 20 minutes after the injection. Although endocystosis occurred in most of the trophozoites 2 minutes after injection of both MPSs, MPS A did not prevent exocystosis and MPS B allowed most of the trophozoites to remain as immature cysts 1 hour after injections. Seventeen hours later, although trophozoite did not grow in povidone-iodine, they grew in both MPSs.
Two major MPSs cannot prevent Acanthamoeba trophozoites from regrowth, povidone-iodine has an enough efficacy for disinfection of Acanthamoeba trophozoites.
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