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Fumika Hotta, Hiroshi Eguchi, Tatsuro Miyamoto, Yoshinori Mitamura, Fumio Saito, Katsuhide Yamazaki; Efficacy of Povidone-iodine For Disinfection of Bacteria in The Contact Lens Storage Cases. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5838.
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© ARVO (1962-2015); The Authors (2016-present)
To examine the disinfectant efficacy of povidone-iodne, hydrogen peroxide, and multipurpose solutions against both planktonic and adherent bacteria in the contact lens storage cases
Six commercially available multipurpose solutions (MPSs), a commercially available hydrogen peroxide, and povidone-iodine were recruited in this study. Twelve clinical isolates (6 strains of Pseudomonas aeruginosa, 1strain of Pseudomonas fluorescens, 1strain of Stenotrophomonas maltophilia, 2 strains of Serratia marcescens, 1strain of Alcaligenes xylosoxidans, 1strain of Chryseobacterum indologenes) were cultivated in Y culture medium and controlled to 107 CFU/ml. The 4mL of disinfectants and 40µL of the controlled liquid were added in the cases. After the stationary cultivation for 4 hours, culture liquid was collected as planktonic bacteria. With regard to adherent bacteria, the 1mL of controlled Y medium was injected into the storage cases and cultivated stationary for 24 hours. The 4mL of disinfectants added into the cases after decantation of Y medium, and inside of the storage cases were scraped by Rasper Check (BD, Japan) after 4 hours. We collected bacteria from Rasper Check as adherent bacteria. Their log reductions of viable bacteria counts by disinfectants were determined in comparison to the loaded counts.
The highest disinfection efficacy against planktonic bacteria of all the strains was found in povidone-iodine. Most MPSs showed > 3log reductions against all the strains. Although povidone-iodine and hydrogen peroxide showed > 4log reduction against adherent bacteria, MPSs showed < 3log reduction against them.
Povidone-iodine has an efficacy for disinfection of adherent bacteria to the contact lens storage cases as well as planktonic bacteria.
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