April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Efficacy of Povidone-iodine For Disinfection of Bacteria in The Contact Lens Storage Cases
Author Affiliations & Notes
  • Fumika Hotta
    Ophthalmology, Tokushima University, Tokushima, Japan
  • Hiroshi Eguchi
    Ophthalmology, Tokushima University, Tokushima, Japan
  • Tatsuro Miyamoto
    Ophthalmology, Tokushima University, Tokushima, Japan
  • Yoshinori Mitamura
    Ophthalmology, Tokushima University, Tokushima, Japan
  • Fumio Saito
  • Katsuhide Yamazaki
  • Footnotes
    Commercial Relationships  Fumika Hotta, None; Hiroshi Eguchi, None; Tatsuro Miyamoto, None; Yoshinori Mitamura, None; Fumio Saito, OPHTECS CORPORATION (E); Katsuhide Yamazaki, OPHTECS CORPORATION (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5838. doi:
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      Fumika Hotta, Hiroshi Eguchi, Tatsuro Miyamoto, Yoshinori Mitamura, Fumio Saito, Katsuhide Yamazaki; Efficacy of Povidone-iodine For Disinfection of Bacteria in The Contact Lens Storage Cases. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5838.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To examine the disinfectant efficacy of povidone-iodne, hydrogen peroxide, and multipurpose solutions against both planktonic and adherent bacteria in the contact lens storage cases

Methods: : Six commercially available multipurpose solutions (MPSs), a commercially available hydrogen peroxide, and povidone-iodine were recruited in this study. Twelve clinical isolates (6 strains of Pseudomonas aeruginosa, 1strain of Pseudomonas fluorescens, 1strain of Stenotrophomonas maltophilia, 2 strains of Serratia marcescens, 1strain of Alcaligenes xylosoxidans, 1strain of Chryseobacterum indologenes) were cultivated in Y culture medium and controlled to 107 CFU/ml. The 4mL of disinfectants and 40µL of the controlled liquid were added in the cases. After the stationary cultivation for 4 hours, culture liquid was collected as planktonic bacteria. With regard to adherent bacteria, the 1mL of controlled Y medium was injected into the storage cases and cultivated stationary for 24 hours. The 4mL of disinfectants added into the cases after decantation of Y medium, and inside of the storage cases were scraped by Rasper Check (BD, Japan) after 4 hours. We collected bacteria from Rasper Check as adherent bacteria. Their log reductions of viable bacteria counts by disinfectants were determined in comparison to the loaded counts.

Results: : The highest disinfection efficacy against planktonic bacteria of all the strains was found in povidone-iodine. Most MPSs showed > 3log reductions against all the strains. Although povidone-iodine and hydrogen peroxide showed > 4log reduction against adherent bacteria, MPSs showed < 3log reduction against them.

Conclusions: : Povidone-iodine has an efficacy for disinfection of adherent bacteria to the contact lens storage cases as well as planktonic bacteria.

Keywords: contact lens 

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