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Yuhua Zhang, Xiaolin Wang, Richard A. Myers, John J. Alexander, Tong Ye, Austin Roorda, Paul D. Gamlin; Broad-Spectrum Adaptive Optics Scanning Laser Ophthalmoscopy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5869.
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To develop an adaptive optics scanning laser ophthalmoscope (AOSLO) that uses a supercontinuum laser and a custom built avalanche photodiodes (APD) with enhanced near-infrared (IR) spectral responsivity, and to apply this instrument to study the spectroscopic reflectance of the cone photoreceptors.
A quasi continuous wave (CW) single mode supercontinuum laser (NKT Photonics A/S, Denmark) that provides light over a spectral range of 500 - 2400 nm was employed as the light source for the AOSLO. A programmable dual-channel acousto-optical tunable filter (AOTF) was used to either continuously tune a single wavelength or simultaneously select multiple wavelengths over the spectral range of 500-1100 nm. The light was fed into the AOSLO through ultra-broadband single mode crystal fibers. A custom APD module with 20 MHz bandwidth, enhanced responsivity in the near-IR region and 4 mm2 active area was developed for photon detection.
Through the AOTF, crystal fiber and AOSLO optics, the broadband light source was able to deliver light with adequate power at any specific wavelength over 500 - 1100 nm to the eye, thus, allowing the retina to be imaged with high quality. The new APD detector presented robust spectral response over the spectral range 500 - 1060 nm; in particular, at 1060 nm, the APD demonstrated superior performance compared with other photodetector options. We have obtained high-resolution spectral retinal images in a living macaque eye.
The AOSLO, with only one broadband light source and a custom-made APD detector, was able to acquire a full range of spectral retinal images in the living eye. In-vivo study of the spectral reflectance of the photoreceptors and other retinal structures is underway.
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