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Yasuhiro Ito, Shintaro Nakao, Souska Zandi, Hidenobu Tanihara; Adiponectin Suppresses Adherent Leukocytes in Retinal Vessels Induced by High-Fat Diet. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5945.
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© ARVO (1962-2015); The Authors (2016-present)
Adiponectin, one of adipocytokines, acts as an endogenous antiinflammatory modulator in diabetes and atherosclerosis. However, the effect of adiponectin in diabetic retnopathy remains to be fully elucidated. We investigated whether adiponectin deficiency might promote the development of diabetic retinopathy under the western-style high-fat diet condition using adiponectin deficiency mice.
Adiponectin deficiency mice and the controls were fed high-fat diet for 1 month. We extracted protein from retinas of those mice and measured the concentration of intercellular adhesion molecule-1 (ICAM-1) using sandwich enzyme-linked immunosorbent assay (ELISA). The retinal vasculature and adherent leukocytes were stained by perfusion labeling with fluorescein isothiocyanate (FITC)-coupled concanavalin A lectin (ConA) for quantification of retinal adherent leukocytes.
ICAM-1 expression in retina was significantly suppressed in adiponectin-deficient mice compared with wild-type mice fed a high-fat diet. A significant increase of adherent leukocytes in retinal vessels was observed in the adiponectin-deficient mice compared with wild-type mice fed high-fat diet.
Adiponectin suppresses ICAM-1 expression and adherent leukocytes in retina induced by high-fat diet. These findings indicate a novel mechanism of suppression of development of diabetic retinopathy induced by western-style high-fat diet.
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