April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
AlphaB-crystallin in Fibrovascular Membrane of Human Proliferative Diabetic Retinopathy
Author Affiliations & Notes
  • Zhenyu Dong
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Satoru Kase
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Ryo Ando
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Junichi Fukuhara
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Anton Lennikov
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Atsuhiro Kanda
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Miyuki Murata
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Kousuke Noda
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Wataru Saito
    Department of Ophthalmology,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Susumu Ishida
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Scienece,
    Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Footnotes
    Commercial Relationships  Zhenyu Dong, None; Satoru Kase, None; Ryo Ando, None; Junichi Fukuhara, None; Anton Lennikov, None; Atsuhiro Kanda, None; Miyuki Murata, None; Kousuke Noda, None; Wataru Saito, None; Susumu Ishida, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5948. doi:https://doi.org/
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      Zhenyu Dong, Satoru Kase, Ryo Ando, Junichi Fukuhara, Anton Lennikov, Atsuhiro Kanda, Miyuki Murata, Kousuke Noda, Wataru Saito, Susumu Ishida; AlphaB-crystallin in Fibrovascular Membrane of Human Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5948. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Recently, we reported that alphaB-crystallin, a molecular chaperone to vascular endothelial growth factor (VEGF), prevented VEGF protein from degradation, consequently promoting angiogenesis (Kase et al, Blood 2010). In this study, we examined the expression of alphaB-crystallin and its co-localization with VEGF in fibrovascular membranes (FVMs) of human proliferative diabetic retinopathy (PDR).

Methods: : Ten FVMs, surgically excised from patients with PDR, were analyzed in this study. As a control, a normal retina obtained by orbital exenteration in a 50-year-old patient due to squamous cell carcinoma invading the orbit without diabetes mellitus was used. The tissues were fixed with paraformaldehyde, embedded in paraffin, and were subjected to immunohistochemistry for alphaB-crystallin, VEGF, and CD31.

Results: : AlphaB-crystallin expression was found in all the FVMs examined. Immunolocalization of alphaB-crystallin was detected in the cytoplasm of CD31-posivtive endothelial cells in the FVMs, but not in the normal retina. Furthermore, alphaB-crystallin was co-localized with VEGF expression in these FVMs.

Conclusions: : AlphaB-crystallin was expressed and co-localized with VEGF in the FVMs obtained from PDR patients. Our data suggest that alphaB-crystallin plays a role in the formation of FVM in PDR, functioning as a molecular chaperone for VEGF.

Keywords: diabetic retinopathy • immunohistochemistry • retinal neovascularization 
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