April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Accessory Lacrimal Tissue Gene Expression
Author Affiliations & Notes
  • Ramesh M. Singa
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago Ear and Eye Infirmary, Chicago, Illinois
  • Vinay K. Aakalu
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago Ear and Eye Infirmary, Chicago, Illinois
  • Amy Lin
    Department of Pathology, University of Illinois at Chicago, Chicago, Illinois
  • Pete Setabutr
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago Ear and Eye Infirmary, Chicago, Illinois
  • Sandeep Jain
    Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago Ear and Eye Infirmary, Chicago, Illinois
  • Footnotes
    Commercial Relationships  Ramesh M. Singa, None; Vinay K. Aakalu, None; Amy Lin, None; Pete Setabutr, None; Sandeep Jain, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 5993. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Ramesh M. Singa, Vinay K. Aakalu, Amy Lin, Pete Setabutr, Sandeep Jain; Accessory Lacrimal Tissue Gene Expression. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5993.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose:
 

Extensive research has shown that pancreatic exocrine tissueand salivary glandular tissue contains slow cycling subsetsof precursor cells which may represent a source of tissue forex vivo expansion and therapeutic application. The present studyis focused on studying the gene expression of accessory lacrimaltissue with the intent of identifying cells which express precursorcell markers.

 
Methods:
 

With IRB approval, conjunctival specimens were obtained frompatients undergoing Müller Muscle-Conjunctival Resection(MMCR) at the Illinois Ear and Eye Infirmary. Accessory lacrimalglands were identified and processed into a tissue microarray(TMA). PDX-1 and nestin antibodies (markers associated withmultipotent cells in exocrine tissue) were applied to severalsections of the TMA and confocal microscopy was used to evaluatefor fluorescence after secondary antibody application. A sectionof the TMA was stained with hematoxylin and eosin and used asa map to characterize the histopathology of tissue.

 
Results:
 

Slides stained with nestin and PDX-1 demonstrated immunofluoroescencein accessory lacrimal gland tissue. Affinityfor nestin (first image) and PDX-1 (second image) in accessorylacrimal gland acini, as seen by rhodamine red stain. 

 

 
Conclusions:
 

Cells in the accessory lacrimal gland tissue that may representprecursor cells as indicated by nestin and PDX-1 marker fluorescencewere identified. Future goals include isolation and culturingof these nestin-and PDX-1-expressing precursor cells.

 
Keywords: lacrimal gland • gene/expression • microscopy: light/fluorescence/immunohistochemistry 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×