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Ramesh M. Singa, Vinay K. Aakalu, Amy Lin, Pete Setabutr, Sandeep Jain; Accessory Lacrimal Tissue Gene Expression. Invest. Ophthalmol. Vis. Sci. 2011;52(14):5993.
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Extensive research has shown that pancreatic exocrine tissueand salivary glandular tissue contains slow cycling subsetsof precursor cells which may represent a source of tissue forex vivo expansion and therapeutic application. The present studyis focused on studying the gene expression of accessory lacrimaltissue with the intent of identifying cells which express precursorcell markers.
With IRB approval, conjunctival specimens were obtained frompatients undergoing Müller Muscle-Conjunctival Resection(MMCR) at the Illinois Ear and Eye Infirmary. Accessory lacrimalglands were identified and processed into a tissue microarray(TMA). PDX-1 and nestin antibodies (markers associated withmultipotent cells in exocrine tissue) were applied to severalsections of the TMA and confocal microscopy was used to evaluatefor fluorescence after secondary antibody application. A sectionof the TMA was stained with hematoxylin and eosin and used asa map to characterize the histopathology of tissue.
Slides stained with nestin and PDX-1 demonstrated immunofluoroescencein accessory lacrimal gland tissue.
Affinityfor nestin (first image) and PDX-1 (second image) in accessorylacrimal gland acini, as seen by rhodamine red stain.
Cells in the accessory lacrimal gland tissue that may representprecursor cells as indicated by nestin and PDX-1 marker fluorescencewere identified. Future goals include isolation and culturingof these nestin-and PDX-1-expressing precursor cells.
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