April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Expression of Receptors for Luteinizing Hormone-Releasing Hormone (LH-RH) in Human Uveal Melanomas
Zita Steiber; Andrea Treszl; Gabor Olah; Armin Buglyo; Bernadett Rozsa; Andras Berta; Gabor Halmos
Author Affiliations & Notes
  • Zita Steiber
    Ophthalmology,
    Biopharmacy,
    University of Debrecen, Debrecen, Hungary
  • Andrea Treszl
    Biopharmacy,
    University of Debrecen, Debrecen, Hungary
  • Gabor Olah
    Biopharmacy,
    University of Debrecen, Debrecen, Hungary
  • Armin Buglyo
    Biopharmacy,
    University of Debrecen, Debrecen, Hungary
  • Bernadett Rozsa
    Biopharmacy,
    University of Debrecen, Debrecen, Hungary
  • Andras Berta
    Ophthalmology,
    University of Debrecen, Debrecen, Hungary
  • Gabor Halmos
    Biopharmacy,
    University of Debrecen, Debrecen, Hungary
  • Footnotes
    Commercial Relationships  Zita Steiber, None; Andrea Treszl, None; Gabor Olah, None; Armin Buglyo, None; Bernadett Rozsa, None; Andras Berta, None; Gabor Halmos, None
  • Footnotes
    Support  Hungarian Scientific Research Fund (OTKA) K 81596 (G.H.) and TAMOP 4.2.1./B-09/1/KONV-2010-0007 project (G.H.), Zoltan Magyary Postdoctoral Fellowship supported by EEA Grants and Norway Grants (A.T.)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6021. doi:
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      Zita Steiber, Andrea Treszl, Gabor Olah, Armin Buglyo, Bernadett Rozsa, Andras Berta, Gabor Halmos; Expression of Receptors for Luteinizing Hormone-Releasing Hormone (LH-RH) in Human Uveal Melanomas. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6021.

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Abstract

Purpose: : Treatment options available for patients with advanced uveal melanoma are limited. The presence of luteinizing hormone-releasing hormone (LH-RH) receptors has been demonstrated in various human cancers. Consequently, we investigated the expression of LH-RH ligand and the presence of receptors for LH-RH in tissue samples and cell lines of human uveal melanoma as potential molecular targets for cancer therapy.

Methods: : The expression of LH-RH ligand and LH-RH receptor transcript forms were investigated by RT-PCR using gene specific primers in a panel of human uveal melanoma specimens and 3 cell lines. The presence of LH-RH receptor protein was evaluated by Western blotting.

Results: : The expression of mRNA for LH-RH receptor transcript forms was detected in 17 out of the 38 tissue specimens (47%) and in 2 of 3 cell lines tested. mRNA for LH-RH ligand could be detected in 76% of tissue samples (29 out of 38) and in all 3 cancer cell lines. No PCR products for pituitary type of LH-RH receptors were found. The presence of specific, full lenght LH-RH receptor protein was demonstrated in tumor specimens by Western blotting.

Conclusions: : We were able to detect the expression of LH-RH and transcript forms of LH-RH receptors in human uveal melanomas. Our results support the merit of further investigation of the expression of LH-RH receptors and its transcript forms in human ophthalmological tumors as well as the application of cytotoxic LH-RH analogs for receptor-based targeted therapy of such malignancies. These observations signify the importance of the development of new LH-RH analogs with potential therapeutical applications.

Keywords: melanoma • uvea • receptors: pharmacology/physiology 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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