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Zita Steiber, Andrea Treszl, Gabor Olah, Armin Buglyo, Bernadett Rozsa, Andras Berta, Gabor Halmos; Expression of Receptors for Luteinizing Hormone-Releasing Hormone (LH-RH) in Human Uveal Melanomas. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6021.
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Treatment options available for patients with advanced uveal melanoma are limited. The presence of luteinizing hormone-releasing hormone (LH-RH) receptors has been demonstrated in various human cancers. Consequently, we investigated the expression of LH-RH ligand and the presence of receptors for LH-RH in tissue samples and cell lines of human uveal melanoma as potential molecular targets for cancer therapy.
The expression of LH-RH ligand and LH-RH receptor transcript forms were investigated by RT-PCR using gene specific primers in a panel of human uveal melanoma specimens and 3 cell lines. The presence of LH-RH receptor protein was evaluated by Western blotting.
The expression of mRNA for LH-RH receptor transcript forms was detected in 17 out of the 38 tissue specimens (47%) and in 2 of 3 cell lines tested. mRNA for LH-RH ligand could be detected in 76% of tissue samples (29 out of 38) and in all 3 cancer cell lines. No PCR products for pituitary type of LH-RH receptors were found. The presence of specific, full lenght LH-RH receptor protein was demonstrated in tumor specimens by Western blotting.
We were able to detect the expression of LH-RH and transcript forms of LH-RH receptors in human uveal melanomas. Our results support the merit of further investigation of the expression of LH-RH receptors and its transcript forms in human ophthalmological tumors as well as the application of cytotoxic LH-RH analogs for receptor-based targeted therapy of such malignancies. These observations signify the importance of the development of new LH-RH analogs with potential therapeutical applications.
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