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Miyuki Kubota, Shunsuke Kubota, Hideyuki Miyashita, Tomohiko Usui, Kazuo Tsubota, Shigeto Shimmura; Hydrogen Rescues Oxidative Stress-induced Angiogenesis In A Mouse Corneal Alkali-burn Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6404.
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Regulation of oxidative stresses is important in some pathological responses. We hypothesized that irrigating with H2 solution, a recently recognized antioxidant will enhance the prophylactic effects against ensuing inflammation and corneal Neovascularization (NV) using an alkali-burn model.
Mice corneas following alkali burns were irrigated with H2 water or PBS for 30 min. Following day, NV areas were visualized by PECAM-1 staining, and inflammatic molecules were measured.
Irrigating with isotonic H2 solution also significantly reduced ROS associated DHE fluorescence (p<0.05). H2 water also had significantly smaller NV areas (p< 0.01) that were associated with lower VEGF (p< 0.01) and MCP-1 (p<0.01) protein levels via NF-ΚB inactivation (p< 0.05) compared to PBS alone irrigation.
Immediate action to quench reactive oxygen species can inhibit angiogenesis. Compared to using expensive reagents such as antibodies or anti-metabolites, H2 water is inexpensive and safe to use in any clinical setting. We hope that our results will shed light on the importance of anti-oxidant therapy in alkali burns.
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