April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Hydrogen Rescues Oxidative Stress-induced Angiogenesis In A Mouse Corneal Alkali-burn Model
Author Affiliations & Notes
  • Miyuki Kubota
    Ophthalmology, Keio Univ School of Med, Shinjuku-ku, Japan
  • Shunsuke Kubota
    Ophthalmology, Keio Univ School of Med, Shinjuku-ku, Japan
  • Hideyuki Miyashita
    Ophthalmology, Keio Univ School of Med, Shinjuku-ku, Japan
  • Tomohiko Usui
    Ophthalmology, Univ of Tokyo Sch of Med, Tokyo, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio Univ School of Med, Shinjuku-ku, Japan
  • Shigeto Shimmura
    Ophthalmology, Keio Univ School of Med, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships  Miyuki Kubota, None; Shunsuke Kubota, None; Hideyuki Miyashita, None; Tomohiko Usui, None; Kazuo Tsubota, None; Shigeto Shimmura, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6404. doi:
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    • Get Citation

      Miyuki Kubota, Shunsuke Kubota, Hideyuki Miyashita, Tomohiko Usui, Kazuo Tsubota, Shigeto Shimmura; Hydrogen Rescues Oxidative Stress-induced Angiogenesis In A Mouse Corneal Alkali-burn Model. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6404.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Regulation of oxidative stresses is important in some pathological responses. We hypothesized that irrigating with H2 solution, a recently recognized antioxidant will enhance the prophylactic effects against ensuing inflammation and corneal Neovascularization (NV) using an alkali-burn model.

Methods: : Mice corneas following alkali burns were irrigated with H2 water or PBS for 30 min. Following day, NV areas were visualized by PECAM-1 staining, and inflammatic molecules were measured.

Results: : Irrigating with isotonic H2 solution also significantly reduced ROS associated DHE fluorescence (p<0.05). H2 water also had significantly smaller NV areas (p< 0.01) that were associated with lower VEGF (p< 0.01) and MCP-1 (p<0.01) protein levels via NF-ΚB inactivation (p< 0.05) compared to PBS alone irrigation.

Conclusions: : Immediate action to quench reactive oxygen species can inhibit angiogenesis. Compared to using expensive reagents such as antibodies or anti-metabolites, H2 water is inexpensive and safe to use in any clinical setting. We hope that our results will shed light on the importance of anti-oxidant therapy in alkali burns.

Keywords: cornea: basic science • neovascularization • oxidation/oxidative or free radical damage 
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