April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Differential Markers for Cultured Human Corneal Endothelial Cells and Stromal Myofibroblast Cells
Author Affiliations & Notes
  • Masahiro Yamaguchi
    Corneal Regeneration Research, Foundation for Biomedical Research, Kobe, Japan
    Ophthalmology, Juntendo university, Tokyo, Japan
  • Nobuyuki Shima
    Ophthalmology, Foundation for Biomedical Research, Chuo-ku, Japan
  • Miwa Kimoto
    Ophthalmology, Foundation for Biomedical Research, Chuo-ku, Japan
  • Nobuyuki Ebihara
    Ophthalmology, Juntendo university, Tokyo, Japan
  • Akira Murakami
    Ophthalmology, Juntendo university, Tokyo, Japan
  • Satoru Yamagami
    Ophthalmology, Foundation for Biomedical Research, Chuo-ku, Japan
    Ophthalmology, Tokyo Women's Medical University Medical Center East, Tokyo, Japan
  • Footnotes
    Commercial Relationships  Masahiro Yamaguchi, None; Nobuyuki Shima, None; Miwa Kimoto, None; Nobuyuki Ebihara, None; Akira Murakami, None; Satoru Yamagami, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6439. doi:
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      Masahiro Yamaguchi, Nobuyuki Shima, Miwa Kimoto, Nobuyuki Ebihara, Akira Murakami, Satoru Yamagami; Differential Markers for Cultured Human Corneal Endothelial Cells and Stromal Myofibroblast Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6439.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To eliminate the possibility of corneal stromal cell contamination is important for cell preparation in cultured human corneal endothelial cell (HCEC) transplantation. We investigated markers, which exclude stromal myofibroblast cell (MF) from HCEC.

Methods: : HCEC and stromal cells were harvested from donor cornea and cultured separately in serum-containing medium. Candidate markers were screened with microarray assay in the 4th passaged HCEC and MF. Then, selected factors were evaluated in RT-PCR, Western blotting, and immunocytochemistry to determine differential markers for each cell.

Results: : Differential markers of HCEC and MF were respective Integrin alpha3 (ITGA3, CD49c) protein with western blotting/immunocytochemistry and iroquois homeobox 2 (IRX2) gene with RT-PCR.

Conclusions: : ITGA3 protein and IRX2 gene can discriminate between HCEC and MF.

Keywords: cornea: endothelium • cornea: stroma and keratocytes 
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