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Masahiro Yamaguchi, Nobuyuki Shima, Miwa Kimoto, Nobuyuki Ebihara, Akira Murakami, Satoru Yamagami; Differential Markers for Cultured Human Corneal Endothelial Cells and Stromal Myofibroblast Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6439.
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© ARVO (1962-2015); The Authors (2016-present)
To eliminate the possibility of corneal stromal cell contamination is important for cell preparation in cultured human corneal endothelial cell (HCEC) transplantation. We investigated markers, which exclude stromal myofibroblast cell (MF) from HCEC.
HCEC and stromal cells were harvested from donor cornea and cultured separately in serum-containing medium. Candidate markers were screened with microarray assay in the 4th passaged HCEC and MF. Then, selected factors were evaluated in RT-PCR, Western blotting, and immunocytochemistry to determine differential markers for each cell.
Differential markers of HCEC and MF were respective Integrin alpha3 (ITGA3, CD49c) protein with western blotting/immunocytochemistry and iroquois homeobox 2 (IRX2) gene with RT-PCR.
ITGA3 protein and IRX2 gene can discriminate between HCEC and MF.
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