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Holly Simpson, K V. Thomas, H Menegay, B A. Benetz, R A. Schachar, S Raber, M Zhang, J Lass; Comparison of Nidek Automated, Automated Adjusted, and Manual Center Methods of Corneal Endothelial Cell Analysis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6445.
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© ARVO (1962-2015); The Authors (2016-present)
To compare corneal endothelial cell image analysis using the Konan KSS-300 center method and the Nidek automated endothelial analysis system with and without manual correction.
Twenty five randomly selected images of good to excellent quality of the central corneal endothelium were obtained from a sample of images by confocal microscopy performed with a Nidek Confoscan 4 (40x objective) collected as part of a study of the corneal effects of dosing taprenepag isopropyl (PF-04217329) qAM for 14 days. The study included 32 patients (24 non-Japanese and 8 Japanese) with a prior history of glaucoma or ocular hypertension, normal slit lamp exam, and no history of ocular trauma, intraocular surgery or intraocular inflammation. Images were captured at baseline, days 7 and 13 on treatment, and day 35. Identical images were analyzed using the Konan KSS-300 center method and the Nidek automated endothelial analysis system provided by the manufacturer (Nidek Technologies, Inc., Greensboro, NC) with (Nidek automated-adjusted) and without manual correction (Nidek automated). All three methods of image analysis were used to determine endothelial cell density (ECD) as well as coefficient of variation (CV) and % of hexagonal cells (%HEX) and were statistically evaluated for differences by the Student’s paired t-tests.
The mean ECD (± SD) determined using the Nidek automated (2468 ± 384 cells/mm2) image analysis software was higher but not statistically significant when compared to the ECD determined with the Konan KSS- 300 center method (2355 ± 552 cells/mm2). The Nidek automated-adjusted (2346 ± 561 cells/mm2) was equivalent to the KSS-300 center method. The mean CV was significantly higher for Nidek automated analysis method (p= 0.001) while the %HEX was significantly lower (p= 0.01) when compared to the Konan method. The Nidek automated-adjusted CV (p= 0.27) and %HEX (p= 0.64) was equivalent to the KSS-300 center method.
The Nidek automated endothelial image analysis software with and without manual editing (automated-adjusted) produces results comparable to the manual Konan KSS-300 center method for determination of ECD but requires manual adjustment for other morphometric parameters.
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