April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Endothelial Toxicity of Genipin vs UV Riboflavin
Author Affiliations & Notes
  • Marcel Y. Avila
    Ophthalmology, Universidad Nacional de Colombia, Bogota DC, Colombia
  • Vannesa A. Gerena
    Ophthalmology, Universidad Nacional de Colombia, Bogota DC, Colombia
  • Footnotes
    Commercial Relationships  Marcel Y. Avila, None; Vannesa A. Gerena, None
  • Footnotes
    Support  Facultad de Medicina Universidad Nacional de Colombia Departamento de Cirugia
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6468. doi:
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      Marcel Y. Avila, Vannesa A. Gerena; Endothelial Toxicity of Genipin vs UV Riboflavin. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6468.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To evaluate corneal endothelial toxicity of corneal crosslinkers

Methods: : Fresh enucleated porcine eyes with clear corneas were crosslinked with genipin 0.25% (n=14) , UV-Riboflavin (n=14) Glutaraldehide 0.1% (n=14) and control SSB (n=14) for 25 minutes. Descemet layer with endothelial cells were insolated and stained with alizarin red to evaluate endothelial changes seeing cellular area, number of cells by mm2 and endothelial cell loss. Cells were phtographed and measured with Image J (NIH ). Statistical analysis of the data was done using analysis of variance (ANOVA) and the Kruskal-Wallis test was used when significative diference was present using Sigma Stat sofware. Mean values are ± SE

Results: : Endothelial cell damage was induced with all crosslinkers Genipin 9.4±1.5 %, UV riboflavin 10.35±1.7%, Glutaraldehide 40.16± 6.23% and control 5.91±1.8% with no diference between Genipin Vs UV riboflavin Vs Control. The numbers of cells by mm2 were genipin 2987±80 cells/mm2, UV riboflavin 2526±109 cells/mm2, Glutaraldehide 2108±153 cells /mm2 and control 2790±50 cells /mm2 with significative diference between genipin vs UV riboflavin (p 0.008) and between genipin, UV riboflavin vs glutaraldehide. Cellular area was genipin 277±8 microns/cells, UV riboflavin 296±9 microns /cell, glutaraldehide 326±16.6 microns/cell and control 288±7 microns/cell. In morphological analysis ocasional cells with edema and with granules were observed in the UV riboflavin group and celular lysis was observed in glutaraldehide group.

Conclusions: : Genipin showed a slightly lower endothelial toxicity compared with UV riboflavin, showing to be a promising crosslinker for corneal ectasia treatment

Keywords: cornea: endothelium • drug toxicity/drug effects • keratoconus 

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