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Nerida Cole, Linda L. Garthwaite, Mark D. Willcox; Effects of Multipurpose Disinfecting Solution Excipients on Corneal Cell Physiology and Cytokine Production. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6485.
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The use of certain multipurpose disinfecting solutions (MPDS) with certain silicone hydrogel contact lenses has been associated with corneal infiltrative events in recent articles. This study examines the contributions of the excipients nonaoyl EDTA (nEDTA) and propylene glycol (PG) on cell numbers, cell metabolic activity, and cell membrane integrity, as well as the production of cytokines by human corneal limbal epithelial cells (HCLE).
HCLE were exposed to dilutions of two MPDS (1-20%), nEDTA (0.01 - 0.2%) or PG (0.2 -5 %) for 2, 6 or 18 hours. Cells were enumerated using Cyquant, cell metabolism was quantified using MTT assay, and cell membrane integrity studied using LIVE/DEAD staining and confocal microscopy. Cytokines released into the culture supernatant were measured by ELISA
Exposure of HCLE to either MPDS resulted in decreases in cell numbers at concentrations of MPDS >10%. Both MPDS slightly reduced cell metabolic activity. Exposure to MPDS containing nEDTA and PG resulted in a significant (p<0.01) increase in cell membrane perturbation, especially at a concentration of 20%. When tested in isolation, nEDTA did not affect cell numbers or cell membranes even at the highest concentration used (0.2%), although it did slightly reduce cell metabolic activity at concentrations >0.05% (p=0.05). PG reduced cell numbers and metabolic activity at its highest concentration (5%; p<0.02) and increased cell membrane perturbation at 5% concentration, although this concentration was above that found in 20% MPDS. The nEDTA/PG containing MPDS (at 20%) resulted in significantly increased production of IL-8 (108.1±12.5 pg/cell) and IL-6 (55±6.3 pg/cell), but addition of nEDTA or PG alone did not increase cytokine production
The MPDS containing nEDTA/PG reduced cell numbers, increased cell membrane permeability and increased cytokine (IL-8/IL-6) production. However, exposing cells to the individual excipients did not replicate the results for the MPDS solution, suggesting that combinations of excipients should be tested.
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