April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Effects of Multipurpose Disinfecting Solution Excipients on Corneal Cell Physiology and Cytokine Production
Author Affiliations & Notes
  • Nerida Cole
    Brien Holden Vision Institute, Kensington, Australia
    School of Optometry and Vision Science, University of New South Wales, Kensington, NSW, Australia
  • Linda L. Garthwaite
    Brien Holden Vision Institute, Kensington, Australia
  • Mark D. Willcox
    Brien Holden Vision Institute, Kensington, Australia
    School of Optometry and Vision Science, University of New South Wales, Kensington, NSW, Australia
  • Footnotes
    Commercial Relationships  Nerida Cole, Bausch and Lomb (F); Linda L. Garthwaite, Bausch and Lomb (F); Mark D. Willcox, Bausch and Lomb (F)
  • Footnotes
    Support  Funded by a grant from Bausch and Lomb Incorporated and by the Brien Holden Vision Institute.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 6485. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Nerida Cole, Linda L. Garthwaite, Mark D. Willcox; Effects of Multipurpose Disinfecting Solution Excipients on Corneal Cell Physiology and Cytokine Production. Invest. Ophthalmol. Vis. Sci. 2011;52(14):6485.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The use of certain multipurpose disinfecting solutions (MPDS) with certain silicone hydrogel contact lenses has been associated with corneal infiltrative events in recent articles. This study examines the contributions of the excipients nonaoyl EDTA (nEDTA) and propylene glycol (PG) on cell numbers, cell metabolic activity, and cell membrane integrity, as well as the production of cytokines by human corneal limbal epithelial cells (HCLE).

Methods: : HCLE were exposed to dilutions of two MPDS (1-20%), nEDTA (0.01 - 0.2%) or PG (0.2 -5 %) for 2, 6 or 18 hours. Cells were enumerated using Cyquant, cell metabolism was quantified using MTT assay, and cell membrane integrity studied using LIVE/DEAD staining and confocal microscopy. Cytokines released into the culture supernatant were measured by ELISA

Results: : Exposure of HCLE to either MPDS resulted in decreases in cell numbers at concentrations of MPDS >10%. Both MPDS slightly reduced cell metabolic activity. Exposure to MPDS containing nEDTA and PG resulted in a significant (p<0.01) increase in cell membrane perturbation, especially at a concentration of 20%. When tested in isolation, nEDTA did not affect cell numbers or cell membranes even at the highest concentration used (0.2%), although it did slightly reduce cell metabolic activity at concentrations >0.05% (p=0.05). PG reduced cell numbers and metabolic activity at its highest concentration (5%; p<0.02) and increased cell membrane perturbation at 5% concentration, although this concentration was above that found in 20% MPDS. The nEDTA/PG containing MPDS (at 20%) resulted in significantly increased production of IL-8 (108.1±12.5 pg/cell) and IL-6 (55±6.3 pg/cell), but addition of nEDTA or PG alone did not increase cytokine production

Conclusions: : The MPDS containing nEDTA/PG reduced cell numbers, increased cell membrane permeability and increased cytokine (IL-8/IL-6) production. However, exposing cells to the individual excipients did not replicate the results for the MPDS solution, suggesting that combinations of excipients should be tested.

Keywords: cytokines/chemokines • cornea: epithelium • contact lens 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×